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Literature summary for 2.4.1.255 extracted from

  • Shen, D.L.; Gloster, T.M.; Yuzwa, S.A.; Vocadlo, D.J.
    Insights into O-linked N-acetylglucosamine (O-GlcNAc) processing and dynamics through kinetic analysis of O-GlcNAc transferase and O-GlcNAcase activity on protein substrates (2012), J. Biol. Chem., 287, 15395-15408.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.255

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 UDP-GlcNAc using calcium/calmodulin-dependent kinase IV as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.0047
-
 UDP-GlcNAc using CARM1 protein as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.0058
-
 UDP-GlcNAc using Nup62 protein as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.007
-
 UDP-GlcNAc using TAB1 protein as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
nucleus
-
 Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens O15294
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with Tau protein Homo sapiens ?
-
 ?
UDP-GlcNAc + calcium/calmodulin-dependent kinase IV
-
 Homo sapiens UDP + N-acetyl-D-glucosaminyl-[calcium/calmodulin-dependent kinase IV]
-
 ?
UDP-GlcNAc + CARM1 protein
-
 Homo sapiens UDP + N-acetyl-D-glucosaminyl-[CARM1 protein]
-
 ?
UDP-GlcNAc + Nup62 protein
-
 Homo sapiens UDP + N-acetyl-D-glucosaminyl-[Nup62 protein]
-
 ?
UDP-GlcNAc + TAB1 protein
-
 Homo sapiens UDP + N-acetyl-D-glucosaminyl-[TAB1 protein]
-
 ?

Synonyms

Synonyms Comment Organism
O-GlcNAc transferase
-
 Homo sapiens
OGT
-
 Homo sapiens
uridine diphosphate-N-acetyl-D-glucosamine:polypeptidyltransferase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000073
-
 UDP-GlcNAc using CARM1 as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.000077
-
 UDP-GlcNAc using calcium/calmodulin-dependent kinase IV as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.00057
-
 UDP-GlcNAc using TAB1 as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.00093
-
 UDP-GlcNAc using Nup62 as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme probably plays a senior role in determining O-linked N-acetylglucosamine levels Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.016
-
 UDP-GlcNAc using CARM1 protein as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.035
-
 UDP-GlcNAc using calcium/calmodulin-dependent kinase IV as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.08
-
 UDP-GlcNAc using TAB1 protein as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens
0.16
-
 UDP-GlcNAc using Nup62 protein as cosubstrate, at 37°C, pH not specified in the publication Homo sapiens