Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.255 extracted from

  • Haltiwanger, R.S.; Blomberg, M.A.; Hart, G.W.
    Glycosylation of nuclear and cytoplasmic proteins. Purification and characterization of a uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase (1992), J. Biol. Chem., 267, 9005-9013.
    View publication on PubMed
Search for more literature for 2.4.1.255

Inhibitors

Inhibitors Comment Organism Structure
CDP
-
 Rattus norvegicus
KCl 50 mM causes 66% inhibition Rattus norvegicus
NaCl 50 mM causes 66% inhibition Rattus norvegicus
thiouridine diphosphate SUDP Rattus norvegicus
UDP UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity Rattus norvegicus
UDP-GalNAc UMP and UDP-GalNAc are 100fold less potent than UDP, UTP, and UDP-GlcNAc Rattus norvegicus
UDP-GlcNAc UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity Rattus norvegicus
UMP UMP and UDP-GalNAc are 100fold less potent than UDP, UTP, and UDP-GlcNAc Rattus norvegicus
UTP UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000545
-
 UDP-GlcNAc extremely high affinity Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
soluble
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ manganese choride 5 mM, essential for binding, although divalent cations are not required for activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 2 * 110000, 1 * 78000 (alpha2beta) Rattus norvegicus
110000
-
 2 * 110000, 1 * 78000 (alpha2beta) Rattus norvegicus
340000
-
 on molecular sieve chromatography Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography, at pH 6.0-6.5 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00111
-
 last purification step Rattus norvegicus

Storage Stability

Storage Stability Organism
Purified enzyme is stable and can be stored for several months at -20°C with negligible loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-GlcNAc + YSDSPSTST highly specific synthetic peptide assay, affinity of the enzyme for UDP-GlcNAc is high and for the peptide substrate, YSDSPSTST, is only moderate Rattus norvegicus UDP + ?
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 2 * 110000, 1 * 78000 (alpha2beta) Rattus norvegicus

Synonyms

Synonyms Comment Organism
O-GlcNAc transferase
-
 Rattus norvegicus
uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 about Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 activity remaining fairly high up to pH 7.5 while dropping rapidly below 6 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00018
-
-
 Rattus norvegicus UDP-GlcNAc
0.0002
-
-
 Rattus norvegicus UDP
0.0002
-
-
 Rattus norvegicus UTP
0.016
-
-
 Rattus norvegicus UDP-GalNAc
0.018
-
-
 Rattus norvegicus UDP
0.019
-
-
 Rattus norvegicus UMP