Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bellis perennis |
Protein Variants | Comment | Organism |
---|---|---|
D152A | mutation decreases the activity with cyanidin 3-O-beta-D-glucoside to less than 15% of wild type | Bellis perennis |
N123A | mutation decreases the activity with cyanidin 3-O-beta-D-glucoside to less than 15% of wild type | Bellis perennis |
R25G | mutant enzyme exhibits only 0.5% to 2.5% of wild-type activity with UDP-D-glucuronate, but shows a 3fold increase in activity with UDP-D-glucose | Bellis perennis |
R25K | mutant enzyme exhibits only 0.5% to 2.5% of wild-type activity with UDP-D-glucuronate, but shows a 3fold increase in activity with UDP-D-glucose | Bellis perennis |
R25S | mutant enzyme exhibits only 0.5% to 2.5% of wild-type activity with UDP-D-glucuronate, but shows a 3fold increase in activity with UDP-D-glucose | Bellis perennis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
cyanidin 3-O-beta-glucoside | pH 7.5, 30°C, wild-type enzyme | Bellis perennis | |
1 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, mutant enzyme I187A | Bellis perennis | |
1.1 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, wild-type enzyme | Bellis perennis | |
1.4 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, mutant enzyme R25S | Bellis perennis | |
1.6 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, mutant enzyme N123A | Bellis perennis | |
1.9 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, mutant enzyme L148A | Bellis perennis | |
2.1 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, mutant enzyme R25G | Bellis perennis | |
2.7 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, mutant enzyme D152A | Bellis perennis | |
7 | - |
UDP-beta-D-glucuronate | pH 7.5, 30°C, mutant enzyme R25K | Bellis perennis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bellis perennis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bellis perennis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-beta-D-glucuronate + cyanidin 3-O-beta-D-glucoside | highly specific with respect to the sugar donor UDP-GlcUA. Activity with other activated sugars such as UDP-Glc, UDP-Gal, and UDP-Xyl is very low. Wild-type enzyme activity toward delphinidin-3-O-glucoside is only 5% to 10% of the activity with cyanidin 3-O-beta-D-glucoside. A few specific amino acid residues as well as the overall size and shape of the acceptor pocket define substrate specificity | Bellis perennis | UDP + cyanidin 3-O-beta-(2-O-beta-D-glucuronosyl)-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BpUGT94B1 | - |
Bellis perennis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bellis perennis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | relative kcat-values for wild-type enzyme and mutant enzymes | Bellis perennis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bellis perennis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | relative kcat/KM values for wild-type enzyme and mutant enzymes | Bellis perennis |