Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dodecylmaltoside | maximal activity in the presence of dodecylmaltoside at a concentration of 33.3 critical micelle concentration | Xanthomonas campestris | |
Triton X-100 | maximal activity at 133 critical micelle concentration or 32 mM or 2%, representing about 45% of that observed with dodecylmaltoside | Xanthomonas campestris |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Xanthomonas campestris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | monotopic membrane protein | Xanthomonas campestris | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE, recombinant protein | Xanthomonas campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | Q56775 | - |
- |
Storage Stability | Organism |
---|---|
-20°C, purified protein at 5 mg/ml is stable for at least 5 months | Xanthomonas campestris |
4°C, purified protein at 5 mg/ml is stable for at least 1 month at 4°C | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | ADP-mannose can act as a mannose donor, yielding around 35% of the reaction product obtained by using GDP-mannose | Xanthomonas campestris | ADP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? | |
GDP-alpha D-glucose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | less than 15% of the reaction product obtained by using GDP-mannose | Xanthomonas campestris | GDP + D-Glc-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? | |
GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | enzyme is a nonprocessive beta-mannosyltransferase catalyzing the transfer of a mannose residue from GDP-Man to glucuronic acid-beta-1,2-mannose-alpha-1,3-glucose-beta-1,4-glucose-PP-polyisoprenyl to form the lipid-linked pentasaccharide | Xanthomonas campestris | GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE, recombinant protein | Xanthomonas campestris |
Synonyms | Comment | Organism |
---|---|---|
gumI | - |
Xanthomonas campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Xanthomonas campestris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
about 80% of maximum activity | Xanthomonas campestris |
60 | - |
almost complete loss of activity | Xanthomonas campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Xanthomonas campestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Xanthomonas campestris |