Cloned (Comment) | Organism |
---|---|
construction of Mycobacterium tuberculosis with two copies of mshA | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | Mycobacterium tuberculosis | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | Mycobacterium tuberculosis Erdman | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
Erdman | - |
Mycobacterium tuberculosis Erdman | - |
Erdman | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + 1D-myo-inositol 3-phosphate = 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis | Mycobacterium tuberculosis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate | Mycobacterium tuberculosis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis | Mycobacterium tuberculosis Erdman | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate | Mycobacterium tuberculosis Erdman | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? |
General Information | Comment | Organism |
---|---|---|
malfunction | inability to disrupt the mshA gene in Mycobacterium tuberculosis containing a single copy of mshA. Directed knock-out of the mshA gene in Mycobacterium tuberculosis Erdman is only possible when a second copy of mshA is first incorporated into the chromosome. Bacteria with only a single copy of mshA that grew after mutagenesis produced normal levels of mycothiol | Mycobacterium tuberculosis |