Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermoproteus tenax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Thermoproteus tenax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
UDP-glucose | in 50 mM HEPES/KOH (pH 7.0), with 20 mM MgCl2, at 70°C | Thermoproteus tenax | |
3.57 | - |
D-glucose | in 50 mM HEPES/KOH (pH 7.0), with 20 mM MgCl2, at 70°C | Thermoproteus tenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Thermoproteus tenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44400 | - |
subunit, calculated from amino acid sequence | Thermoproteus tenax |
47000 | - |
subunit, SDS-PAGE | Thermoproteus tenax |
100000 | - |
native enzyme, gel filtration | Thermoproteus tenax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoproteus tenax | - |
- |
- |
Thermoproteus tenax Kra 1 / DSM 2078 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Thermoproteus tenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose | 10% activity with ADP-glucose compared to UDP-glucose | Thermoproteus tenax | ADP + alpha,alpha-1,1-trehalose | - |
r | |
ADP-alpha-D-glucose + D-glucose | 10% activity with ADP-glucose compared to UDP-glucose | Thermoproteus tenax Kra 1 / DSM 2078 | ADP + alpha,alpha-1,1-trehalose | - |
r | |
additional information | no activity with maltose and glucose 1-phosphate | Thermoproteus tenax | ? | - |
? | |
additional information | no activity with maltose and glucose 1-phosphate | Thermoproteus tenax Kra 1 / DSM 2078 | ? | - |
? | |
UDP-glucose + D-glucose | clear preference for UDP-glucose over ADP-glucose | Thermoproteus tenax | alpha,alpha-1,1-trehalose + UDP | - |
r | |
UDP-glucose + D-glucose | clear preference for UDP-glucose over ADP-glucose | Thermoproteus tenax Kra 1 / DSM 2078 | alpha,alpha-1,1-trehalose + UDP | - |
r |
Synonyms | Comment | Organism |
---|---|---|
trehalose glycosyltransferring synthase | - |
Thermoproteus tenax |
TreT | - |
Thermoproteus tenax |