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Literature summary for 2.4.1.245 extracted from

  • Ryu, S.I.; Park, C.S.; Cha, J.; Woo, E.J.; Lee, S.B.
    A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: Molecular cloning and characterization (2005), Biochem. Biophys. Res. Commun., 329, 429-436.
    View publication on PubMed
Search for more literature for 2.4.1.245

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with His6-tag Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48197
-
 x * 48197, calculated, x * 49871, MALDI-TOF mass spectrometry of recombinant His-tagged protein Pyrococcus horikoshii
49871
-
 x * 48197, calculated, x * 49871, MALDI-TOF mass spectrometry of recombinant His-tagged protein Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58762
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + D-glucose
-
 Pyrococcus horikoshii ADP + alpha,alpha-1,1-trehalose
-
 ?
GDP-glucose + D-glucose
-
 Pyrococcus horikoshii alpha,alpha-1,1-trehalose + GDP
-
 ?
additional information UDP-glucose is as efficient as ADP-glucose and GDP-glucose Pyrococcus horikoshii ?
-
 ?
UDP-glucose + D-glucose
-
 Pyrococcus horikoshii alpha,alpha-1,1-trehalose + UDP
-
 ?

Subunits

Subunits Comment Organism
? x * 48197, calculated, x * 49871, MALDI-TOF mass spectrometry of recombinant His-tagged protein Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 1 h, 25% residual activity Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pyrococcus horikoshii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 highly stable within this range Pyrococcus horikoshii