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Literature summary for 2.4.1.242 extracted from

  • Nyvall, P.; Pelloux, J.; Davies, H.V.; Pedersen, M.; Viola, R.
    Purification and characterization of a novel starch synthase selective for uridine 5'-diphosphate glucose from the red alga Gracilaria tenuistipitata (1999), Planta, 209, 143-152.
    View publication on PubMed
Search for more literature for 2.4.1.242

Activating Compound

Activating Compound Comment Organism Structure
citrate stimulates the UDP-glucose utilizing enzyme Agarophyton tenuistipitatum
UDP product inhibition of the UDP-glucose utilizing enzyme Agarophyton tenuistipitatum

General Stability

General Stability Organism
rapid loss of activity of purified UDP-glucose utilizing enzyme within hours Agarophyton tenuistipitatum

Inhibitors

Inhibitors Comment Organism Structure
additional information UDP-glucose utilizing activity is sensitive to proteolytic inhibition during extraction, while the ADP-glucose utilizing activity is unaffected Agarophyton tenuistipitatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Agarophyton tenuistipitatum
0.17
-
 glycogen UDP-glucose utilizing enzyme in presence of 0.5 M citrate Agarophyton tenuistipitatum
0.52
-
 amylopectin UDP-glucose utilizing enzyme in presence of 0.5 M citrate Agarophyton tenuistipitatum
0.69
-
 glycogen UDP-glucose utilizing enzyme in absence of citrate Agarophyton tenuistipitatum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol starch granule-bound Agarophyton tenuistipitatum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 4 * 145000, UDP-glucose utilizing enzyme, SDS-PAGE Agarophyton tenuistipitatum
580000
-
 UDP-glucose utilizing enzyme, gel filtration Agarophyton tenuistipitatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-glucose + (1,4-alpha-glucosyl)n Agarophyton tenuistipitatum ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme ADP + (1,4-alpha-glucosyl)n+1
-
 ?
UDP-glucose + (1,4-alpha-glucosyl)n Agarophyton tenuistipitatum UDP-glucose is the preferred glucosyl donor, utilized by the UDP-glucose utilizing enzyme UDP + (1,4-alpha-glucosyl)n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Agarophyton tenuistipitatum
-
 red alga
-

Oxidation Stability

Oxidation Stability Organism
UDP-glucose utilizing activity is sensitive to oxidative inhibition during extraction, while the ADP-glucose utilizing activity is unaffected Agarophyton tenuistipitatum

Purification (Commentary)

Purification (Comment) Organism
ADP-glucose utilizing activity partially, UDP-glucose utilizing activity 368.7fold to homogeneity Agarophyton tenuistipitatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 UDP-glucose utilizing and ADP-glucose utilizing activities in different cell fractions and with different primer substrate, overview Agarophyton tenuistipitatum
0.229
-
 purified UDP-glucose utilizing enzyme Agarophyton tenuistipitatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + (1,4-alpha-glucosyl)n ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme Agarophyton tenuistipitatum ADP + (1,4-alpha-glucosyl)n+1
-
 ?
ADP-glucose + (1,4-alpha-glucosyl)n ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme and UDP-glucose utilizing enzyme, the latter shows 3.8% activity compared to UDP-glucose Agarophyton tenuistipitatum ADP + (1,4-alpha-glucosyl)n+1
-
 ?
ADP-glucose + amylopectin
-
 Agarophyton tenuistipitatum ADP + ?
-
 ?
CDP-glucose + (1,4-alpha-glucosyl)n UDP-glucose utilizing enzyme, 2.5% of the activity with UDP-glucose Agarophyton tenuistipitatum CDP + ?
-
 ?
GDP-glucose + (1,4-alpha-glucosyl)n UDP-glucose utilizing enzyme, 28.7% of the activity with UDP-glucose Agarophyton tenuistipitatum GDP + ?
-
 ?
additional information the UDP-glucose utilizing enzyme shows broad primer specificity Agarophyton tenuistipitatum ?
-
 ?
NDP-glucose + glycogen
-
 Agarophyton tenuistipitatum ?
-
 ?
UDP-galactose + (1,4-alpha-glucosyl)n UDP-glucose utilizing enzyme, 10.1% of the activity with UDP-glucose Agarophyton tenuistipitatum UDP + ?
-
 ?
UDP-glucose + (1,4-alpha-glucosyl)n UDP-glucose is the preferred glucosyl donor, utilized by the UDP-glucose utilizing enzyme Agarophyton tenuistipitatum UDP + (1,4-alpha-glucosyl)n+1
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 145000, UDP-glucose utilizing enzyme, SDS-PAGE Agarophyton tenuistipitatum

Synonyms

Synonyms Comment Organism
UDPGlc: starch synthase
-
 Agarophyton tenuistipitatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 8.1 with substrate glycogen Agarophyton tenuistipitatum
8.1
-
 with substrate amylopectin Agarophyton tenuistipitatum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 with substrate amylopectin, sharp drop of activity below pH 6.5 and more gradually above pH 8.0 Agarophyton tenuistipitatum
7 9 with substrate amylopectin, sharp drop of activity below pH 7.0 and above pH 9.0 Agarophyton tenuistipitatum