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Literature summary for 2.4.1.242 extracted from
- Evidence that the waxy protein of pea (Pisum sativum L.) is not the major starch-granule-bound starch synthase (1990), Planta, 182, 599-604.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | stimulates the enzyme activity in presence of a primer | Pisum sativum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibition of activity by a specific antibody | Pisum sativum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77000 | - |
x * 77000, SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + (1,4-alpha-glucosyl)n | Pisum sativum | the waxy protein and the granule-bound starch synthase are not identical | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially from dried starch granules isolated from developing embryos, solubilization with alpha-amylase, and anion chromatography | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | developing | Pisum sativum | - |
| seed | starch granule | Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + (1,4-alpha-glucosyl)n | - |
Pisum sativum | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
| ADP-glucose + (1,4-alpha-glucosyl)n | the waxy protein and the granule-bound starch synthase are not identical | Pisum sativum | ADP + (1,4-alpha-glucosyl)n+1 | - |
? | |
| additional information | most effective primer of the reaction is amylopectin, 16% activity with glycogen compared to amylopectin | Pisum sativum | ? | - |
? | |
| UDP-glucose + (1,4-alpha-glucosyl)n | 10% of the activity with ADP-glucose | Pisum sativum | UDP + (1,4-alpha-glucosyl)n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 77000, SDS-PAGE | Pisum sativum |
| More | minor bands of 57 kDa and 92 kDa are recognized by the specific antibody | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| starch-granule-bound starch synthase | - |
Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Pisum sativum |
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Release 2023.1 (January 2023)
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