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Literature summary for 2.4.1.234 extracted from

  • Taylor, L.P.; Miller, K.D.
    The use of a photoactivatable kaempferol analogue to probe the role of flavonol 3-O-galactosyltransferase in pollen germination (2002), Adv. Exp. Med. Biol., 505, 41-50.
    View publication on PubMed
Search for more literature for 2.4.1.234

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Petunia sp.

Inhibitors

Inhibitors Comment Organism Structure
2-(3-benzoylphenyl)-3,5,7-trihydroxychromen-4-one irreversible inhibitor, the effects of the inhibitor are mediated by UV-A light treatment, binding characteristics, kaempferol protects against inhibition Petunia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 kaempferol
-
 Petunia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information localization of F3GalTase in pollen Petunia sp.
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + kaempferol Petunia sp. role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen UDP + kaempferol 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r
UDP-galactose + quercetin Petunia sp. role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen UDP + quercetin 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r

Organism

Organism UniProt Comment Textmining
Petunia sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
gametophyte gametophyte-specific enzyme Petunia sp.
-
pollen CMF Petunia sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + 2-(3-benzoylphenyl)-3,5,7-trihydroxychromen-4-one 20-30% of the activity with kaempferol as substrate Petunia sp. UDP + 2-(3-benzoylphenyl)-3,5,7-trihydroxychromen-4-one galactoside
-
 ?
UDP-galactose + kaempferol role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen Petunia sp. UDP + kaempferol 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r
UDP-galactose + kaempferol F3GalTase is a highly discriminating and efficient enzyme Petunia sp. UDP + kaempferol 3-O-beta-D-galactoside
-
 r
UDP-galactose + quercetin role in pollen germination, F3GalTase is a gametophyte-specific enzyme, that controls the accumulation of glycosylated flavonols in pollen Petunia sp. UDP + quercetin 3-O-beta-D-galactoside endogenous product in pollen, which stimulates pollen germination r
UDP-galactose + quercetin F3GalTase is a highly discriminating and efficient enzyme Petunia sp. UDP + quercetin 3-O-beta-D-galactoside
-
 r

Subunits

Subunits Comment Organism
monomer
-
 Petunia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reverse activity Petunia sp.
8
-
 forward activity Petunia sp.