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Literature summary for 2.4.1.231 extracted from

  • Nakai, H.; Petersen, B.O.; Westphal, Y.; Dilokpimol, A.; Abou Hachem, M.; Duus, J.?.; Schols, H.A.; Svensson, B.
    Rational engineering of Lactobacillus acidophilus NCFM maltose phosphorylase into either trehalose or kojibiose dual specificity phosphorylase (2010), Protein Eng. Des. Sel., 23, 781-787.
    View publication on PubMed
Search for more literature for 2.4.1.231

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
22
-
 alpha,alpha-trehalose loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C Lactobacillus acidophilus
53
-
 alpha,alpha-trehalose loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C Lactobacillus acidophilus

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus
-
-
-
Lactobacillus acidophilus NCFM
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + phosphate
-
 Lactobacillus acidophilus alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 ?
alpha,alpha-trehalose + phosphate
-
 Lactobacillus acidophilus NCFM alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 ?
additional information the maltose phosphorylase loop 3 variants TP2 and TP3 are capable of phosphorolysing trehalose (alpha, alpha1) as well as maltose (alpha-1,4),but show no activity towards kojibiose (alpha-1,2), nigerose (alpha-1,3) or isomaltose (alpha-1,6). The catalytic efficiency of TP3 towards trehalose is 19fold higher than of TP2 Lactobacillus acidophilus ?
-
 ?
additional information the maltose phosphorylase loop 3 variants TP2 and TP3 are capable of phosphorolysing trehalose (alpha, alpha1) as well as maltose (alpha-1,4),but show no activity towards kojibiose (alpha-1,2), nigerose (alpha-1,3) or isomaltose (alpha-1,6). The catalytic efficiency of TP3 towards trehalose is 19fold higher than of TP2 Lactobacillus acidophilus NCFM ?
-
 ?

Synonyms

Synonyms Comment Organism
TP2 a loop 3 variant of Lactobacillus acidophilus maltose phosphorylase Lactobacillus acidophilus
TP3 a loop 3 variant of Lactobacillus acidophilus maltose phosphorylase Lactobacillus acidophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Lactobacillus acidophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 50
-
 Lactobacillus acidophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
 alpha,alpha-trehalose loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C Lactobacillus acidophilus
2.7
-
 alpha,alpha-trehalose loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C Lactobacillus acidophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 6.7
-
 Lactobacillus acidophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.2 8.9
-
 Lactobacillus acidophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0062
-
 alpha,alpha-trehalose loop 3 variant TP2, in 40 mM phosphate-citrate pH 6.2 at 45°C Lactobacillus acidophilus
0.12
-
 alpha,alpha-trehalose loop 3 variant TP3, in 40 mM phosphate-citrate pH 6.2 at 45°C Lactobacillus acidophilus