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Literature summary for 2.4.1.231 extracted from
- The phosphate site of trehalose phosphorylase from Schizophyllum commune probed by site-directed mutagenesis and chemical rescue studies (2008), FEBS J., 275, 903-913.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| guanidine | no effect on wild-type but guanidine causes a 45fold enhancement of mutant R507A activity | Schizophyllum commune |  |
| propargylamine | no effect on wild-type but propagylamine causes a 23fold enhancement of mutant K512A activity | Schizophyllum commune | |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K512A | kcat/Km (phosphate): 0.0014/Msec, vanadate shows a weaker inhibitory effect compared to wild-type, propargylamine leads to a 23fold increase in activity, phosphate 50 mM enhances basal hydrolase activity | Schizophyllum commune |
| R507A | Km (phosphate) 103 mM, kcat/Km (phosphate): 0.0004/Msec, vanadate shows no inhibitory effect, guanidine leads to a 45fold increase in activity | Schizophyllum commune |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | phosphate 50 mM causes total supression in wild-type but enhances mutant K512 activity | Schizophyllum commune | |
| vanadate | strong inhibitor for wildtype ScTPase and weak inhibitor for mutant K512A but no mutant R507A is not inhibited at all | Schizophyllum commune | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | mutant K512A (phosphate): no saturation, Kcat/Km (phosphate): 0.0014/Msec | Schizophyllum commune | |
| 0.8 | - |
phosphate | wild-type, Kcat/Km (phosphate): 21000/Msec | Schizophyllum commune | |
| 103 | - |
phosphate | mutant R507A, Kcat/Km (phosphate): 0.0004/Msec | Schizophyllum commune | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizophyllum commune | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + phosphate | - |
Schizophyllum commune | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ScTPase | - |
Schizophyllum commune |
| trehalose phosphorylase | - |
Schizophyllum commune |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Schizophyllum commune |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
assay at | Schizophyllum commune |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
vanadate | wild-type | Schizophyllum commune | |
| 0.298 | - |
vanadate | mutant K512A | Schizophyllum commune | |
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