Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | stimulates phosphorolytic activity at 10 mM | Agaricus bisporus | |
ATP | stimulates synthetic activity at 10 mM | Agaricus bisporus |
General Stability | Organism |
---|---|
highly unstable during purification, glycerol, phosphate, dithiothreitol and PMSF stabilize | Agaricus bisporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | 88% inhibition at 10 mM | Agaricus bisporus | |
ADP | 34% inhibition at 10 mM | Agaricus bisporus | |
ATP | inhibits trehalose degradation, but stimulates synthetic activity | Agaricus bisporus | |
CoCl2 | 39% inhibition at 1 mM | Agaricus bisporus | |
CuSO4 | 37% inhibition at 1 mM | Agaricus bisporus | |
phosphate | inhibits synthetic activity | Agaricus bisporus | |
validamycin A | 99% inhibition at 10 mM | Agaricus bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
phosphate | 30°C, pH 7 | Agaricus bisporus | |
6.3 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 7 | Agaricus bisporus | |
24 | - |
alpha-D-glucose | 30°C, pH 7 | Agaricus bisporus | |
61 | - |
alpha,alpha-trehalose | 30°C, pH 7 | Agaricus bisporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
4 * 61000, SDS-PAGE | Agaricus bisporus |
240000 | - |
native PAGE, gel filtration | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + phosphate | Agaricus bisporus | key role in trehalose metabolism | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
alpha,alpha-trehalose + phosphate | Agaricus bisporus Horst U1 | key role in trehalose metabolism | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Agaricus bisporus Horst U1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Agaricus bisporus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate | mechanism | Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Agaricus bisporus | - |
mycelium | - |
Agaricus bisporus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.35 | - |
- |
Agaricus bisporus |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 6 months | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + phosphate | - |
Agaricus bisporus | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
alpha,alpha-trehalose + phosphate | key role in trehalose metabolism | Agaricus bisporus | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
alpha,alpha-trehalose + phosphate | - |
Agaricus bisporus Horst U1 | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
alpha,alpha-trehalose + phosphate | key role in trehalose metabolism | Agaricus bisporus Horst U1 | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 61000, SDS-PAGE | Agaricus bisporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
almost complete loss of activity after 60 min | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | synthesis of trehalose | Agaricus bisporus |
6 | 7.5 | phosphorolytic reaction | Agaricus bisporus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
phosphate | 30°C, pH 7 | Agaricus bisporus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Agaricus bisporus | isoelectric focusing between pH 3-9 and pH 3-10 | 4.8 | 4.7 |