Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | competitive with respect to phosphate | Schizophyllum commune |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
2.8 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
39 | - |
alpha-D-glucose | 30°C, pH 6.6 | Schizophyllum commune | |
91 | - |
alpha,alpha-trehalose | 30°C, pH 6.6 | Schizophyllum commune |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Schizophyllum commune |
61000 | - |
SDS-PAGE | Schizophyllum commune |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + phosphate | Schizophyllum commune | - |
alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizophyllum commune | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Schizophyllum commune |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate | mechanism | Schizophyllum commune |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.85 | - |
- |
Schizophyllum commune |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + arsenate | - |
Schizophyllum commune | alpha-D-glucose 1-arsenate + alpha-D-glucose | - |
r | |
alpha,alpha-trehalose + phosphate | - |
Schizophyllum commune | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE, gel filtration | Schizophyllum commune |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize | Schizophyllum commune |
20 | - |
unstable, loses activity within a few hours, glycerol, PEG 4000 or trehalose stabilize | Schizophyllum commune |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
10 | - |
alpha-D-glucose | 30°C, pH 6.6 | Schizophyllum commune | |
14 | - |
phosphate | 30°C, pH 6.6 | Schizophyllum commune | |
15 | - |
alpha,alpha-trehalose | 30°C, pH 6.6 | Schizophyllum commune |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
reverse reaction | Schizophyllum commune |
6.6 | - |
forward reaction | Schizophyllum commune |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 6.6 | decrease in enzyme activity below pH 6.2 and above pH 6.6 | Schizophyllum commune |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
NaCl | 30°C, pH 6.6 | Schizophyllum commune |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Schizophyllum commune | isoelectric focusing between pH 4 and pH 6.5 | - |
4.8 |