Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij-35 | slightly stimulating | Dictyostelium sp. | |
dithiothreitol | activates, best at 5 mM | Dictyostelium sp. | |
additional information | requires reducing condition | Dictyostelium sp. | |
NP-40 | slightly stimulating | Dictyostelium sp. | |
Triton X-100 | slightly stimulating | Dictyostelium sp. | |
Tween 20 | stimulates, less efficient than Tween 80 | Dictyostelium sp. | |
Tween 80 | stimulates | Dictyostelium sp. | |
Uracil | stimulates the purified enzyme at 0.1 mM | Dictyostelium sp. |
General Stability | Organism |
---|---|
Tween 20 and Tween 80 stabilize | Dictyostelium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | weak inhibition | Dictyostelium sp. | |
Ca2+ | - |
Dictyostelium sp. | |
CTP | weak inhibition | Dictyostelium sp. | |
EDTA | complete inhibition at 5 mM | Dictyostelium sp. | |
K+ | weak | Dictyostelium sp. | |
Mn2+ | - |
Dictyostelium sp. | |
additional information | Skp-1-protein isoform containing unhydroxylated proline143 is probably inhibitory | Dictyostelium sp. | |
Na+ | inhibitory at concentrations above 0.05 M | Dictyostelium sp. | |
UDP | - |
Dictyostelium sp. | |
UMP | best inhibitor | Dictyostelium sp. | |
UTP | - |
Dictyostelium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
UDP-GlcNAc | pH 7.8, 30°C | Dictyostelium sp. | |
0.00056 | - |
[Skp1-protein]-trans-4-hydroxy-L-proline | pH 7.8, 30°C | Dictyostelium sp. | |
1.6 | - |
hydroxyproline-peptide | pH 7.8, 30°C | Dictyostelium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Dictyostelium sp. | 5829 | - |
additional information | no activity in the particulate fraction | Dictyostelium sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, best at 10 mM | Dictyostelium sp. | |
additional information | divalent cations required | Dictyostelium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Dictyostelium sp. |
51000 | - |
1 * 51000, SDS-PAGE | Dictyostelium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | Dictyostelium sp. | - |
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
130000fold | Dictyostelium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00023 | - |
purified enzyme | Dictyostelium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + [Skp1-protein]-trans-4-hydroxy-L-proline | - |
Dictyostelium sp. | UDP + [Skp1-protein]-O-(N-acetyl-alpha-D-glucosaminyl)-trans-4-hydroxy-L-proline | - |
? | |
UDP-N-acetylglucosamine + hydroxyproline-peptide | synthetic peptide substrate | Dictyostelium sp. | UDP + (N-acetyl-D-glucosaminyl)hydroxyproline peptide | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | - |
Dictyostelium sp. | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | hydroxyproline143 | Dictyostelium sp. | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | high affinity for UDP-GlcNAc | Dictyostelium sp. | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | recombinant protein substrate, deficient in GlcNAc | Dictyostelium sp. | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 51000, SDS-PAGE | Dictyostelium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Dictyostelium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
partially purified enzyme, unstable | Dictyostelium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Dictyostelium sp. |