Cloned (Comment) | Organism |
---|---|
gene GnT51, DNA sequence determination and analysis, functional expression of wild-type and mutants as fusion proteins with CBD-intein, in Escherichia coli strain ER2566, autocleavage of tag moiety | Dictyostelium discoideum |
Protein Variants | Comment | Organism |
---|---|---|
D102A | site-directed mutagenesis, no remaining activity | Dictyostelium discoideum |
H104D | site-directed mutagenesis, no remaining activity | Dictyostelium discoideum |
K23R | site-directed mutagenesis, unaltered enzyme activity | Dictyostelium discoideum |
L276S | site-directed mutagenesis, 7% remaining activity compared to the wild-type enzyme | Dictyostelium discoideum |
additional information | C-terminal deletion mutants GnT51(1-282) and GnT51(1-369+) show no remaining activity | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Dictyostelium discoideum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29600 | - |
x * 29600, mutant GnT51(1-282), SDS-PAGE | Dictyostelium discoideum |
44000 | - |
x * 44000, mutant GnT51(1-369+), SDS-PAGE | Dictyostelium discoideum |
47500 | - |
x * 47500, wild-type enzyme and mutants K23R, D102A, H104D, and L276S, SDS-PAGE | Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | Dictyostelium discoideum | - |
UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | Q8T1C6 | gene GnT51 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and mutants from Escherichia coli as CBD-intein tagged proteins, to near homogeneity | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | - |
Dictyostelium discoideum | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | enzyme attaches the first sugar of pentasaccharide chain | Dictyostelium discoideum | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? | |
UDP-N-acetylglucosamine + [Skp1-protein]-hydroxyproline | hydroxyproline143 | Dictyostelium discoideum | UDP + [Skp1-protein]-O-(N-acetyl-D-glucosaminyl)hydroxyproline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47500, wild-type enzyme and mutants K23R, D102A, H104D, and L276S, SDS-PAGE | Dictyostelium discoideum |
? | x * 44000, mutant GnT51(1-369+), SDS-PAGE | Dictyostelium discoideum |
? | x * 29600, mutant GnT51(1-282), SDS-PAGE | Dictyostelium discoideum |
Synonyms | Comment | Organism |
---|---|---|
GnT51 | - |
Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Dictyostelium discoideum |