KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
lactosylceramide | - |
Rattus norvegicus | |
0.03 | - |
UDP-galactose | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | some activity | Rattus norvegicus | |
Mn2+ | a second divalent cation in the enzyme assay can inhibit the enzyme activity | Rattus norvegicus | |
Ni2+ | some activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE | Rattus norvegicus |
65000 | - |
alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE | Rattus norvegicus |
90000 | - |
corresponding to the Stokes radius of 40 A, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide | Rattus norvegicus | - |
UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | stored at -80°C until use | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00458 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, in 20% glycerol, stored at a concentration greater than 0.1 mg/ml without detectable loss of activity upon repeated freezing and thawing for 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrates: galactosylceramide, glucosylceramide, neolactotetraose, neolactotetraosylceramide | Rattus norvegicus | ? | - |
? | |
UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide | - |
Rattus norvegicus | UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide | - |
? | |
UDP-galactose + lactose | - |
Rattus norvegicus | ? | - |
? | |
UDP-galactose + lactosylceramide | UDP-galactose is the sole sugar donor | Rattus norvegicus | UDP + globotriaosylceramide | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7.4 | - |
Rattus norvegicus |