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Literature summary for 2.4.1.227 extracted from
- The biosynthesis of peptidoglycan lipid-linked intermediates (2008), FEMS Microbiol. Rev., 32, 208-233.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | the activity of MurG is increased in the presence of cardiolipin | Escherichia coli |  |
| DMSO | high concentration (35%) of dimethylsulfoxide is necessary for maximal enzyme activity | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by UMP, nor any other nucleotide diphosphate, UDP-N-acetylgalactosamine fails to show inhibitory activity even at millimolar concentrations | Escherichia coli | |
| ramoplanin | - |
Escherichia coli | |
| UDP | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
biotin-labelled lipid I analogue | in the presence of high concentration (35%) of dimethylsulfoxide | Escherichia coli | |
| 0.037 | - |
biotin-labelled lipid I analogue | in the presence of MgCl2 | Escherichia coli | |
| 0.058 | - |
UDP-N-acetylglucosamine | in the presence of MgCl2 | Escherichia coli | |
| 0.15 | - |
UDP-N-acetylglucosamine | in the presence of high concentration (35%) of dimethylsulfoxide | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37800 | - |
calculated from amino acid sequence | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | UDP-N-acetylgalactosamine shows very little donor activity | Escherichia coli | ? | - |
? | |
| UDP-N-acetylglucosamine + biotin-labelled lipid I analogue | - |
Escherichia coli | ? | - |
? | |
| UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
Escherichia coli | UDP + N-acetylglucosamine-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MurG | - |
Escherichia coli |
| MurG transferase | - |
Escherichia coli |
| translocase II | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
UDP-N-acetylglucosamine | in the presence of MgCl2 | Escherichia coli | |
| 0.93 | - |
UDP-N-acetylglucosamine | in the presence of high concentration (35%) of dimethylsulfoxide | Escherichia coli | |
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