Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chondroitin synthase-1 | co-expressed with chondroitin synthase-1 CSS3 also exhibits polymerase activity | Homo sapiens | |
chondroitin-polymerizing factor | co-expressed with ChPF (chondroitin-polymerizing factor) enzyme shows chondroitin polymerase activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D184A | mutant protein which lacks any glycosyltransferase activity but interacts with other ChSy family members shows that the glycosyltransferase activity of CSGlcA-T plays an important role in chondroitin polymerization | Homo sapiens |
additional information | overexpression of CSGlcA-T increases the amount of chondroitin sulfate in HeLa cells, whereas the RNA interference of CSGlcA-T results in a reduction of the amount of chondroitin sulphate in the cells | Homo sapiens |
additional information | overexpression of CSS3 increases the amount of chondroitin sulfate in HeLa cells, while the RNA interference of CSS3 results in a reduction in the amount of CS in the cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | A2V663 | - |
- |
Purification (Comment) | Organism |
---|---|
a soluble form of CSGlcA-T is generated by replacing the first 57 amino acids of the protein with a cleavable insulin signal sequence and a protein A IgG-binding domain. The soluble protein is expressed in COS-1 cells as a recombinant protein fused with the protein A IgG-binding domain. The fusion protein secreted into the medium is adsorbed onto IgG-Sepharose beads for purification to eliminate endogenous glycosyltransferases, and then the protein-bound beads are used as an enzyme source | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CSGlcA-T exhibits polymerization activity on alpha-thrombomodulin using alpha-thrombomodulin as acceptor incubated with UDP-Gal-NAc and UDP-GlcUA. The chain length of chondroitin formed by the co-expressed proteins ChSy-1,ChSy-2 (CSS3), or ChPF in various combinations is different | Homo sapiens | ? | - |
? | |
additional information | polymerization reactions carried out using alpha-thrombomodulin as an acceptor co-incubated with UDP-GalNAc and UDP-GlcA | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chondroitin sulfate glucoronyltransferase | - |
Homo sapiens |
chondroitin sulfate synthase-3 | enzyme possesses dual enzymatic GalNAcT-II (N-acetylgalactosaminyltransferase-II) and GlcAT-II (glucuronyltransferase-II) activities | Homo sapiens |
chondroitin synthase-3 | - |
Homo sapiens |
ChSy-3 | - |
Homo sapiens |
CSGlcA-T | enzyme posseses weak GlcAT-II and GalNAcT-II activities | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |