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Literature summary for 2.4.1.224 extracted from

  • Senay, C.; Lind, T.; Muguruma, K.; Tone, Y.; Kitagawa, H.; Sugahara, K.; Lidholt, K.; Lindahl, U.; Kusche-Gullberg, M.
    The EXT1/EXT2 tumor suppressors: catalytic activities and role in heparan sulfate biosynthesis (2000), EMBO Rep., 1, 282-286.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.224

Cloned(Commentary)

Cloned (Comment) Organism
expression of full length and truncated form in Pichia pastoris and COS-1 cells, dramatic increase of activity when full length EXT1 and EXT2 are coexpressed, no increase of activity when mixing extract containing EXT1 and EXT2 protein Mus musculus
expression of full length and truncated form in Pichia pastoris and COS-1 cells, dramatic increase of activity when full length EXT1 and EXT2 are coexpressed, no increase of activity when mixing extract containing EXT1 and EXT2 protein Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 SDS-PAGE of expressed EXT1 and EXT2 proteins, weak band at 90000 D Mus musculus
80000
-
 SDS-PAGE of expressed EXT1 and EXT2 proteins, weak band at 90000 D Bos taurus
90000
-
 SDS-PAGE of coexpressed EXT1 and EXT2, no second band detected Mus musculus
90000
-
 SDS-PAGE of coexpressed EXT1 and EXT2, no second band detected Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Mus musculus elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Bos taurus elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 EXT2 protein
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Mus musculus
-
 EXT1 protein
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no activity in yeast
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-linked glycosylation, Endo H treatment does not effect 80000 Da species but reduces size of 90000 Da species that is found mainly when both proteins are coexpressed Mus musculus
glycoprotein N-linked glycosylation, Endo H treatment does not effect 80000 Da species but reduces size of 90000 Da species that is found mainly when both proteins are coexpressed Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information EXT1 and EXT2 proteins also acts as beta(1,4)-glucuronyltransferase (EC 2.4.1.225) Mus musculus ?
-
 ?
additional information EXT1 and EXT2 proteins also acts as beta(1,4)-glucuronyltransferase (EC 2.4.1.225) Bos taurus ?
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Mus musculus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Mus musculus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?

Synonyms

Synonyms Comment Organism
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