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Literature summary for 2.4.1.222 extracted from

  • Pasek, M.; Ramakrishnan, B.; Boeggeman, E.; Mercer, N.; Dulcey, A.E.; Griffiths, G.L.; Qasba, P.K.
    The N-acetyl-binding pocket of N-acetylglucosaminyltransferases also accommodates a sugar analog with a chemical handle at C2 (2012), Glycobiology, 22, 379-388.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.222

Cloned(Commentary)

Cloned (Comment) Organism
expression of MFeng as soluble protein in fusion with galectin-1 from pLgals1-hum-MFng in Escherichia coli Rosetta (DE3) pLysS Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 53000, recombinant galectin-1-hum-MFng fusion protein, SDS-PAGE, x * 33000, recombinant detagged MFng, SDS-PAGE Homo sapiens
53000
-
 x * 53000, recombinant galectin-1-hum-MFng fusion protein, SDS-PAGE, x * 33000, recombinant detagged MFng, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant fusion protein galectin-1-hum-MFng from Escherichia coli strain Rosetta (DE3) pLysS, the fusion protein is cleaved with Tev protease to release the MFng protein, further purification by UDP-agarose chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 free MFng shows a specific activity of 0.5 pmol/min/mg of protein with 100 mM fucose as an acceptor and 0.6 pmol/min/mg of protein with 4 mM 4-nitrophenyl-2-fucose as an acceptor Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information MFng transfers GlcNAc in beta1-3-linkage to O-fucose residues present on the EGF repeats. Free MFng shows a specific activity with fucose and with 4 mM 4-nitrophenyl-2-fucose as acceptors. Transfer of C2-keto-glucose to fucose residues on EGF repeat from factor VII by the hum-MFng, substrate specificity, overview Homo sapiens ?
-
 ?
[factor VII]-fucose + UDP-alpha-D-N-acetylglucosamine residues on EGF repeat from factor VII Homo sapiens [factor VII]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP
-
 ?
[factor VII]-fucose + UDP-alpha-N-acetyl-2-amino-2-deoxy-D-glucose residues on EGF repeat from factor VII Homo sapiens [factor VII]-(3-O-beta-D-N-acetylglucosaminyl)fucose + UDP
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, recombinant galectin-1-hum-MFng fusion protein, SDS-PAGE, x * 33000, recombinant detagged MFng, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
beta1,3-N-acetylglucosaminyltransferase-2
-
 Homo sapiens
Maniac Fringe
-
 Homo sapiens
MFng
-
 Homo sapiens