Cloned (Comment) | Organism |
---|---|
expressed in Drosophila S2 cells | Drosophila melanogaster |
Lunatic Fringe and Manic Fringe expressed in Chinese hamster ovary Lec1 cells | Mammalia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Manganese | - |
Drosophila melanogaster | |
Manganese | - |
Mammalia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Mammalia | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-beta-D-GlcNAc + PCQNGGS(O-beta-L-fucosyl)-CKDQL = UDP + PCQNGGS(O-D-GlcNAcbeta(1-3)-L-Fucbeta)-CKDQL | GlcNAc-transfer activity of Fringe is essential for it to modulate Notch signalling | Drosophila melanogaster |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Lunic Fringe has a higher specific activity than either Drosophila Fringe and Manic Fringe | Drosophila melanogaster |
additional information | - |
Lunic Fringe has a higher specific activity than either Drosophila Fringe and Manic Fringe | Mammalia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epidermal growth factor-like sequence repeats-O-fucose + UDP-GlcNAc | Drosophila Fringe, Lunic Fringe, Manic Fringe | Drosophila melanogaster | ? | - |
? | |
epidermal growth factor-like sequence repeats-O-fucose + UDP-GlcNAc | Drosophila Fringe, Lunic Fringe, Manic Fringe | Mammalia | ? | - |
? | |
additional information | further substrate: recombinant epidermal growth factor repeat from the serum protein factor VII modified with O-linked fucose | Drosophila melanogaster | ? | - |
? | |
additional information | further substrate: recombinant epidermal growth factor repeat from the serum protein factor VII modified with O-linked fucose | Mammalia | ? | - |
? | |
additional information | no substrates: p-nitrophenyl-galactose, p-nitrophenyl-glucose | Drosophila melanogaster | ? | - |
? | |
additional information | no substrates: p-nitrophenyl-galactose, p-nitrophenyl-glucose | Mammalia | ? | - |
? | |
O-linked fucose on the epidermal growth factor-like sequence repeats of Notch + GlcNAc | - |
Drosophila melanogaster | GlcNAc-beta-1,3-fucitol-Notch | - |
? | |
O-linked fucose on the epidermal growth factor-like sequence repeats of Notch + GlcNAc | endogenous Notch1, Manic Fringe | Mammalia | GlcNAc-beta-1,3-fucitol-Notch | - |
? | |
p-nitrophenyl-alpha-L-fucose + UDP-GlcNAc | p-nitrophenyl-alpha-L-fucose structurally mimics O-linked fucose, Lunic Fringe, Manic Fringe, Drosophila Fringe | Drosophila melanogaster | GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP | - |
? | |
p-nitrophenyl-alpha-L-fucose + UDP-GlcNAc | p-nitrophenyl-alpha-L-fucose structurally mimics O-linked fucose, Lunic Fringe, Manic Fringe, Drosophila Fringe | Mammalia | GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP | - |
? | |
UDP-beta-D-GlcNAc + fucosyl-protein | - |
Drosophila melanogaster | UDP + O-beta-D-GlcNAc-fucosyl-protein | - |
? | |
UDP-beta-D-GlcNAc + fucosyl-protein | - |
Mammalia | UDP + O-beta-D-GlcNAc-fucosyl-protein | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay | Drosophila melanogaster |
37 | - |
enzyme assay | Mammalia |