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Literature summary for 2.4.1.222 extracted from

  • Moloney, D.J.; Panin, V.M.; Johnston, S.H.; Chen, J.; Shao, U.; Wilson, R.; Wang, Y.; Stanley, P.; Irvine, K.D.; Haltwanger, R.S.; Vogt, T.F.
    Fringe is a glycosyltransferase that modifies Notch (2000), Nature, 406, 369-375.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Drosophila S2 cells Drosophila melanogaster
Lunatic Fringe and Manic Fringe expressed in Chinese hamster ovary Lec1 cells Mammalia

Metals/Ions

Metals/Ions Comment Organism Structure
Manganese
-
Drosophila melanogaster
Manganese
-
Mammalia

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Mammalia
-
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-beta-D-GlcNAc + PCQNGGS(O-beta-L-fucosyl)-CKDQL = UDP + PCQNGGS(O-D-GlcNAcbeta(1-3)-L-Fucbeta)-CKDQL GlcNAc-transfer activity of Fringe is essential for it to modulate Notch signalling Drosophila melanogaster

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
Lunic Fringe has a higher specific activity than either Drosophila Fringe and Manic Fringe Drosophila melanogaster
additional information
-
Lunic Fringe has a higher specific activity than either Drosophila Fringe and Manic Fringe Mammalia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epidermal growth factor-like sequence repeats-O-fucose + UDP-GlcNAc Drosophila Fringe, Lunic Fringe, Manic Fringe Drosophila melanogaster ?
-
?
epidermal growth factor-like sequence repeats-O-fucose + UDP-GlcNAc Drosophila Fringe, Lunic Fringe, Manic Fringe Mammalia ?
-
?
additional information further substrate: recombinant epidermal growth factor repeat from the serum protein factor VII modified with O-linked fucose Drosophila melanogaster ?
-
?
additional information further substrate: recombinant epidermal growth factor repeat from the serum protein factor VII modified with O-linked fucose Mammalia ?
-
?
additional information no substrates: p-nitrophenyl-galactose, p-nitrophenyl-glucose Drosophila melanogaster ?
-
?
additional information no substrates: p-nitrophenyl-galactose, p-nitrophenyl-glucose Mammalia ?
-
?
O-linked fucose on the epidermal growth factor-like sequence repeats of Notch + GlcNAc
-
Drosophila melanogaster GlcNAc-beta-1,3-fucitol-Notch
-
?
O-linked fucose on the epidermal growth factor-like sequence repeats of Notch + GlcNAc endogenous Notch1, Manic Fringe Mammalia GlcNAc-beta-1,3-fucitol-Notch
-
?
p-nitrophenyl-alpha-L-fucose + UDP-GlcNAc p-nitrophenyl-alpha-L-fucose structurally mimics O-linked fucose, Lunic Fringe, Manic Fringe, Drosophila Fringe Drosophila melanogaster GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP
-
?
p-nitrophenyl-alpha-L-fucose + UDP-GlcNAc p-nitrophenyl-alpha-L-fucose structurally mimics O-linked fucose, Lunic Fringe, Manic Fringe, Drosophila Fringe Mammalia GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP
-
?
UDP-beta-D-GlcNAc + fucosyl-protein
-
Drosophila melanogaster UDP + O-beta-D-GlcNAc-fucosyl-protein
-
?
UDP-beta-D-GlcNAc + fucosyl-protein
-
Mammalia UDP + O-beta-D-GlcNAc-fucosyl-protein
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
enzyme assay Drosophila melanogaster
37
-
enzyme assay Mammalia