Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.221 extracted from

  • Stahl, M.; Uemura, K.; Ge, C.; Shi, S.; Tashima, Y.; Stanley, P.
    Roles of Pofut1 and O-fucose in mammalian Notch signaling (2008), J. Biol. Chem., 283, 13638-13651.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.221

Cloned(Commentary)

Cloned (Comment) Organism
embryonic stem cells transfected with wild-type or mutant R245A Mus musculus

Protein Variants

Protein Variants Comment Organism
R245A is fucosylation-defective, has a dominant negative effect on wild-type Pofut1, since Pofut1 activity in Pofut1-/- cells expressing Pofut1 R245A is markedly reduced in the in vitro assay Mus musculus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell fucosylation-deficient Lec13 chinese hamster ovary cells have wild-type levels of Pofut1 and cell surface Notch receptors Cricetulus griseus
-
embryonic stem cell derived from blastocyst outgrowths Mus musculus
-

Synonyms

Synonyms Comment Organism
Pofut1
-
 Cricetulus griseus
Pofut1
-
 Mus musculus
protein O-fucosyltransferase 1
-
 Cricetulus griseus
protein O-fucosyltransferase 1
-
 Mus musculus

General Information

General Information Comment Organism
malfunction embryonic stem cells lacking Pofut1 are deficient in Notch ligand binding, have wild-type levels of cell surface Notch receptors. Pofut1-/- embryonic stem cells do not bind Notch ligands or exhibit Notch signaling. Overexpression of fucosyltransferase-defective Pofut1 R245A in Pofut1-/- cells partially rescues ligand binding and Notch signaling, but this effect is not specific. Under certain conditions, mammalian Notch receptors can bind Notch ligands and transduce a Notch signal in the absence of Pofut1 and O-fucose Mus musculus
malfunction siRNAs eliminating Pofut1 transcripts in CHO cells. CHO cells deficient in Pofut1 have reduced Notch signaling and ligand binding. Under certain conditions, mammalian Notch receptors can bind Notch ligands and transduce a Notch signal in the absence of Pofut1 and O-fucose Cricetulus griseus
physiological function Notch receptors require Pofut1 for the generation of optimally functional Notch receptors, but Pofut1 is not required for stable cell surface expression of Notch Cricetulus griseus
physiological function Notch receptors require Pofut1 for the generation of optimally functional Notch receptors, but Pofut1 is not required for stable cell surface expression of Notch Mus musculus