Cloned (Comment) | Organism |
---|---|
corresponding gene is expressed in Escherichia coli | Rubrobacter xylanophilus |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the unliganded MpgS and of its complexes with the nucleotide and sugar donors, at 2.2, 2.8 and 2.5 A resolution respectively | Rubrobacter xylanophilus |
Protein Variants | Comment | Organism |
---|---|---|
A164S/A208S/F211Y | MpgS triple mutant engineered to reinforce the hydrogen-bonding network | Rubrobacter xylanophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
GDP-mannose | wild-type | Rubrobacter xylanophilus | |
0.34 | - |
3-phospho-D-glycerate | wild-type, with GDP-mannose | Rubrobacter xylanophilus | |
0.49 | - |
GDP-mannose | A164S/A208S/F211Y mutant | Rubrobacter xylanophilus | |
0.81 | - |
3-phospho-D-glycerate | A164S/A208S/F211Y mutant, with GDP-mannose | Rubrobacter xylanophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ | Rubrobacter xylanophilus | |
Mg2+ | strictly dependent on, effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ | Rubrobacter xylanophilus | |
Mn2+ | effect of divalent cations on MpgS activity decreases in the following order Mg2+, Co2+, Mn2+ | Rubrobacter xylanophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubrobacter xylanophilus | B7SY86 | DSM 9941 | - |
Purification (Comment) | Organism |
---|---|
purified from cell extracts | Rubrobacter xylanophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0232 | - |
with GDP-mannose as a substrate | Rubrobacter xylanophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + 3-phospho-D-glycerate | - |
Rubrobacter xylanophilus | GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mannosyl-3-phosphoglycerate synthase | - |
Rubrobacter xylanophilus |
mannosyl-3-phosphoglycerate synthase/phosphatase | - |
Rubrobacter xylanophilus |
MPGS | GT81 family | Rubrobacter xylanophilus |
MpgS/P | - |
Rubrobacter xylanophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 75 | - |
Rubrobacter xylanophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is thermophilic | Rubrobacter xylanophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Rubrobacter xylanophilus |