Literature summary for 2.4.1.216 extracted from

Carvalho, A.L.; Cardoso, F.S.; Bohn, A.; Neves, A.R.; Santos, H.
Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance (2011), Appl. Environ. Microbiol., 77, 4189-4199.

Search for more literature for 2.4.1.216

Protein Variants

Protein Variants	Comment	Organism
additional information	endogenous genes trePP and pgmB, encoding trehalose 6-phosphate phosphorylase and beta-phosphoglucomutase, respectively, provide the synthesis of trehalose 6-phosphate, which can be transformed into trehalose by transgenically expressed trehalose 6-phosphate phosphatase, the respective gene otsB from the food-grade organism Propionibacterium freudenreichii is expressed in Lactobacillus lactis. Trehalose-producing strains show improved tolerance of 5 to 10fold higher survivability to acid at pH 3.0 and cold shock of 4°C, also a strong improvement in cell survival in response to heat shock of 45°C is achieved, but no protection is rendered against dehydration, phenotype overview	Lactococcus lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis	-	and strain derivatives, overview, gene trePP	-
Lactococcus lactis MG1363	-	and strain derivatives, overview, gene trePP	-

Synonyms

Synonyms	Comment	Organism
TrePP	-	Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Lactococcus lactis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Lactococcus lactis

General Information

General Information	Comment	Organism
physiological function	genes trePP and pgmB, encoding trehalose 6-phosphate phosphorylase and beta-phosphoglucomutase, respectively, enable the synthesis of trehalose 6-phosphate in the organism	Lactococcus lactis

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Release 2023.1 (January 2023)