Literature summary for 2.4.1.214 extracted from

Strasser, R.; Stadlmann, J.; Schaehs, M.; Stiegler, G.; Quendler, H.; Mach, L.; Gloessl, J.; Weterings, K.; Pabst, M.; Steinkellner, H.
Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure (2008), Plant Biotechnol. J., 6, 392-402.

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Application

Application	Comment	Organism
drug development	RNA interference technology obtains a targeted down-regulation of the endogenous beta1,2-xylosyltransferase (XylT) and alpha1,3-fucosyltransferase (FucT) genes, whereby generating RNAi lines which are stable, viable and do not show any obvious phenotype, thus providing a robust tool for the production of therapeutically relevant glycoproteins in plants with a humanized N -glycan structure	Nicotiana benthamiana

Cloned(Commentary)

Cloned (Comment)	Organism
FucT cDNA (414 bp) amplified and cloned into the BamHI site of puc18XTI2 to create puc18Fsi. The antisense fragment amplified and cloned into the KpnI digested vector puc18Fsi to generate puc18Fsias. Subsequently, the 'sense-intron-antisense' cassette excised by XbaI digestion and cloned into XbaI linearized plant expression vector pGA643	Nicotiana benthamiana

Organism

Organism	UniProt	Comment	Textmining
Nicotiana benthamiana	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Nicotiana benthamiana	-

Synonyms

Synonyms	Comment	Organism
alpha1,3-fucosyltransferase	-	Nicotiana benthamiana
FucT	-	Nicotiana benthamiana

Expression

Organism	Comment	Expression
Nicotiana benthamiana	RNAi-targeted down-regulation of the endogenous FucT	down

General Information

General Information	Comment	Organism
physiological function	an incomplete down-regulation of FucT activity is sufficient to allow the generation of immunoglobulin Gs carrying a glycan-optimized structure	Nicotiana benthamiana

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Release 2023.1 (January 2023)