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Literature summary for 2.4.1.214 extracted from
- Molecular cloning and characterization of the Caenorhabditis elegans alpha1,3-fucosyltransferase family (2007), Glycobiology, 17, 586-599.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in COS7 cells, fusion of CFET-1 to green fluorescent protein | Caenorhabditis elegans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activating for CFET-1, 2 and 3, inhibitory for CFET-4 | Caenorhabditis elegans |  |
| Co2+ | activating for CFET-1 and 3, no effect on CFET-2 and 4 | Caenorhabditis elegans | |
| Cu2+ | inhibitory for CFET-1, 2, 3 and 4 | Caenorhabditis elegans | |
| Mg2+ | activating for CFET-1 and 2, inhibitory for CFET-3 and 4 | Caenorhabditis elegans | |
| Mn2+ | activating for CFET-1, inhibitory for CFET-2, 3 and 4 | Caenorhabditis elegans | |
| Ni2+ | inihibitory for CFET-1, 2, 3 and 4 | Caenorhabditis elegans | |
| Zn2+ | activating for CFET 2, inhibitory for CFET-1, 3 and 4 | Caenorhabditis elegans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
SDS-PAGE, Western blot | Caenorhabditis elegans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
wild type and CEFT-1/VC378, CEFT-2/RB511, CEFT-3/RB706,CEFT-4/VC182, and CEFT-5/VC212 deletion mutants | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | CFET-1 | Caenorhabditis elegans | - |
| neuron | CFET-1 | Caenorhabditis elegans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-fucose + Galbeta1-4GlcNAcalpha-p-nitrophenol | CFET-3 required no cations, CFET-4 required divalent cations | Caenorhabditis elegans | Galbeta1-4(Fucalpha1-3)GlcNAcalpha-p-nitrophenol | - |
? | |
| GDP-fucose + Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc | CFET-3 required no cations, CFET-4 required divalent cations | Caenorhabditis elegans | GDP + Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc | - |
? | |
| GDP-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc | LDNT, CFET-2 | Caenorhabditis elegans | GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc | - |
? | |
| GDP-fucose + Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAcbeta1-Asn | CFET-1 | Caenorhabditis elegans | Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)(Fucalpha1-3)GlcNAcb1-Asn | - |
? | |
| GDP-fucose + Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAcbeta1-Asn | CFET-1, Nglycan pentasaccharide core acceptor as substrate | Caenorhabditis elegans | GDP + Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-Asn | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha1,3-fucosyltransferase | - |
Caenorhabditis elegans |
| alpha1,3FTs | - |
Caenorhabditis elegans |
| CEFT-1 | - |
Caenorhabditis elegans |
| CEFT-2 | - |
Caenorhabditis elegans |
| CEFT-3 | - |
Caenorhabditis elegans |
| CEFT-4 | - |
Caenorhabditis elegans |
| CEFT-5 | - |
Caenorhabditis elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
room temperature | Caenorhabditis elegans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 37 | loss of activity at 37°C | Caenorhabditis elegans |
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