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Literature summary for 2.4.1.214 extracted from

  • Paschinger, K.; Staudacher, E.; Stemmer, U.; Fabini, G.; Wilson, I.B.
    Fucosyltransferase substrate specificity and the order of fucosylation in invertebrates (2005), Glycobiology, 15, 463-474.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme, in Pichia pastoris strain GS115 Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates, at 10 mM MnCl2 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schistosoma sp. the native enzyme accepts core alpha1,6-fucosylated glycans as substrates for core alpha1,3-fucosylation prior Lewis-type fucosylation, strict order of fucosylation events, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Schistosoma sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Drosophila melanogaster
additional information
-
-
Schistosoma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,6)GlcNAc preferred substrate Drosophila melanogaster GDP + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)(Fucalpha1,6)GlcNAc
-
?
GDP-L-fucose + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4GlcNAc
-
Drosophila melanogaster GDP + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)GlcNAc
-
?
GDP-L-fucose + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4GlcNAc
-
Schistosoma sp. GDP + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)GlcNAc
-
?
additional information substrate specificity Schistosoma sp. ?
-
?
additional information the native enzyme accepts core alpha1,6-fucosylated glycans as substrates for core alpha1,3-fucosylation prior Lewis-type fucosylation, strict order of fucosylation events, overview Schistosoma sp. ?
-
?
additional information substrate specificity, no activity with GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)GlcNAc, the recombinant enzyme accepts core alpha1,6-fucosylated glycans as substrates for core alpha1,3-fucosylation prior Lewis-type fucosylation, strict order of fucosylation events, overview Drosophila melanogaster ?
-
?

Synonyms

Synonyms Comment Organism
core alpha1,3-fucosyltransferase
-
Drosophila melanogaster
core alpha1,3-fucosyltransferase
-
Schistosoma sp.
FucTA
-
Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Drosophila melanogaster