Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme, in Pichia pastoris strain GS115 | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates, at 10 mM MnCl2 | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Schistosoma sp. | the native enzyme accepts core alpha1,6-fucosylated glycans as substrates for core alpha1,3-fucosylation prior Lewis-type fucosylation, strict order of fucosylation events, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Schistosoma sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Drosophila melanogaster |
additional information | - |
- |
Schistosoma sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,6)GlcNAc | preferred substrate | Drosophila melanogaster | GDP + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)(Fucalpha1,6)GlcNAc | - |
? | |
GDP-L-fucose + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4GlcNAc | - |
Drosophila melanogaster | GDP + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)GlcNAc | - |
? | |
GDP-L-fucose + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4GlcNAc | - |
Schistosoma sp. | GDP + GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)GlcNAc | - |
? | |
additional information | substrate specificity | Schistosoma sp. | ? | - |
? | |
additional information | the native enzyme accepts core alpha1,6-fucosylated glycans as substrates for core alpha1,3-fucosylation prior Lewis-type fucosylation, strict order of fucosylation events, overview | Schistosoma sp. | ? | - |
? | |
additional information | substrate specificity, no activity with GlcNAcbeta1,2Manalpha1,6(GlcNAcbeta1,2Manalpha1,3)Manbeta1,4GlcNAcbeta1,4(Fucalpha1,3)GlcNAc, the recombinant enzyme accepts core alpha1,6-fucosylated glycans as substrates for core alpha1,3-fucosylation prior Lewis-type fucosylation, strict order of fucosylation events, overview | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
core alpha1,3-fucosyltransferase | - |
Drosophila melanogaster |
core alpha1,3-fucosyltransferase | - |
Schistosoma sp. |
FucTA | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Drosophila melanogaster |