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Literature summary for 2.4.1.213 extracted from

  • Novak, J.F.; Stirnberg, M.; Roenneke, B.; Marin, K.
    A novel mechanism of osmosensing, a salt-dependent protein-nucleic acid interaction in the cyanobacterium Synechocystis species PCC 6803 (2011), J. Biol. Chem., 286, 3235-3241.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.213

Cloned(Commentary)

Cloned (Comment) Organism
gene ggpS, expression of Strep-tagged GgpS in Synechocystis sp. Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
Nucleic acids GgpS is inhibited by nucleic acids in a sequence- and length-independent manner in vitro and in vivo, it binds DNA, RNA, and heparin via a salt-dependent electrostatic interaction with the negatively charged backbone of the polyanions. Non-competitive inhibition. The inhibition is completely abolished by 20 mM NaCl Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 ADP-glucose pH 8.0, 30°C Synechocystis sp.
0.71
-
 sn-glycerol 3-phosphate pH 8.0, 30°C Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-glucose + sn-glycerol 3-phosphate Synechocystis sp.
-
 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 gene ggpS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + sn-glycerol 3-phosphate
-
 Synechocystis sp. 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate + ADP
-
 ?
ADP-glucose + sn-glycerol 3-phosphate reaction of second order Synechocystis sp. 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate + ADP
-
 ?

Synonyms

Synonyms Comment Organism
GGPS
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Synechocystis sp.

General Information

General Information Comment Organism
metabolism GgpS is the key enzyme of the glucosylglycerol pathway The intermediate is dephosphorylated by the second enzyme of the pathway, the glucosylglycerol-phosphate phosphatase, GgpP Synechocystis sp.
physiological function the moderately halotolerant cyanobacterium Synechocystis sp. PCC 6803 synthesizes glucosylglycerol as a compatible solute. Mechanism of activity regulation by the nonspecific salt-dependent binding of an enzyme to nucleic acids, overview Synechocystis sp.