Literature summary for 2.4.1.212 extracted from

Vigetti, D.; Genasetti, A.; Karousou, E.; Viola, M.; Clerici, M.; Bartolini, B.; Moretto, P.; De Luca, G.; Hascall, V.C.; Passi, A.
Modulation of hyaluronan synthase activity in cellular membrane fractions (2009), J. Biol. Chem., 284, 30684-30694.

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Activating Compound

Activating Compound	Comment	Organism	Structure
interleukin 1beta	activation of enzyme in plasma membrane fraction	Homo sapiens
phorbol 12-myristate 13-acetate	activation of enzyme in plasma membrane fraction	Homo sapiens
platelet-derived growth factor BB	activation of enzyme in plasma membrane fraction	Homo sapiens
tunicamycin	significant increase in HAS activity in the cytosolic membrane fraction after tunicamycin treatment	Homo sapiens

Application

Application	Comment	Organism
analysis	quantification of newly synthesized hyaluronan by polyacrylamide gel electrophoresis of fluorophore-labeled saccharides and high performance liquid chromatography. The method measures HAS activity in the plasma membrane fraction and also in the cytosolic membranes. The technique is used to evaluate the effects of 4-methylumbeliferone, phorbol 12-myristate 13-acetate, interleukin 1, platelet-derived growth factor BB, and tunicamycin on HAS activities	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: 4-methylumbeliferone	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	fraction containing membrane proteins from the endoplasmic reticulum and Golgi apparatus	Homo sapiens	16020	-
plasma membrane	-	Homo sapiens	5886	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	HAS activity can be modulated by post-translational modification, such as phosphorylation and N-glycosylation	Homo sapiens
phosphoprotein	HAS activity can be modulated by post-translational modification, such as phosphorylation and N-glycosylation	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
ECV-304 cell	-	Homo sapiens	-
U-937 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-D-glucosamine + UDP-D-glucuronate	-	Homo sapiens	[beta-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + n UDP	-	?

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Release 2023.1 (January 2023)