Literature summary for 2.4.1.212 extracted from

Kumari, K.; Baggenstoss, B.A.; Parker, A.L.; Weigel, P.H.
Mutation of two intramembrane polar residues conserved within the hyaluronan synthase family alters hyaluronan product size (2006), J. Biol. Chem., 281, 11755-11760.

Search for more literature for 2.4.1.212

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant enzymes in Escherichia coli. SeHAS(E327Q) and seHAS(E327K) are expressed at low levels, whereas seHAS(E327D) and the Lys48 mutants are expressed well	Streptococcus dysgalactiae subsp. equisimilis

Protein Variants

Protein Variants	Comment	Organism
E327D	the specific enzyme activity relative to wild type enzyme is 38%. Mutant enzyme synthesizes hyaluronan of smaller weight-average molar mass than wild-type enzyme	Streptococcus dysgalactiae subsp. equisimilis
E327K	the specific enzyme activity relative to wild type enzyme is 0.16%. Mutant enzyme synthesizes hyaluronan of smaller weight-average molar mass than wild-type enzyme	Streptococcus dysgalactiae subsp. equisimilis
E327K/K48E	the specific enzyme activity near wild-type level. Mutant enzyme synthesizes hyaluronan of smaller weight-average molar mass than wild-type enzymel	Streptococcus dysgalactiae subsp. equisimilis
E327Q	the specific enzyme activity relative to wild type enzyme is 26%. Mutant enzyme synthesizes hyaluronan of smaller weight-average molar mass than wild-type enzyme	Streptococcus dysgalactiae subsp. equisimilis
K48E	the specific enzyme activity relative to wild type enzyme is 7%. Mutant enzyme synthesizes hyaluronan of smaller weight-average molar mass than wild-type enzyme	Streptococcus dysgalactiae subsp. equisimilis
K48R	the specific enzyme activity relative to wild type enzyme is 17%. Mutant enzyme synthesizes hyaluronan of smaller weight-average molar mass than wild-type enzyme	Streptococcus dysgalactiae subsp. equisimilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Streptococcus dysgalactiae subsp. equisimilis	-	-	-

Synonyms

Synonyms	Comment	Organism
seHAS	-	Streptococcus dysgalactiae subsp. equisimilis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)