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Literature summary for 2.4.1.212 extracted from

  • Itano, N.; Sawai, T.; Atsumi, F.; Miyaishi, O.; Taniguchi, S.; Kannagi, R.; Hamaguchi, M.; Kimata, K.
    Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation (2004), J. Biol. Chem., 279, 18679-18687.
    View publication on PubMed
Search for more literature for 2.4.1.212

Cloned(Commentary)

Cloned (Comment) Organism
HAS1 DNA and amino acid sequence determination and analysis, oncogenic malignant transformation of 3Y1 fibroblasts with v-sre and/or v-fos, or v-HA-ras Rattus norvegicus
HAS2 DNA and amino acid sequence determination and analysis, oncogenic malignant transformation of 3Y1 fibroblasts with v-sre and/or v-fos, or v-HA-ras Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information expression of isozyme HAS1 is increased after oncogenic malignant transformation with v-sre and/or v-fos, no increase after transformation with v-HA-ras, introduction of isozyme HAS1 promotes the growth of subcutaneous tumors dependent on hyaluronan synthesis level Rattus norvegicus
additional information expression of isozyme HAS2 is increased after oncogenic malignant transformation with v-HA-ras, v-sre and/or v-fos, antisense repression of HAS2 expression leads to reduced hyaluronan synthesis and reduced tumorigenicity in the peritoneum, introduction of isozyme HAS2 promotes the growth of subcutaneous tumors dependent on hyaluronan synthesis level Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus isozyme expression and effects on tumor development and growth in rats, overview ?
-
 ?
additional information Rattus norvegicus isozyme expression and effects on tumor development and growth in rats, overview, repression of HAS2 expression leads to reduced hyaluronan synthesis and reduced tumorigenicity in the peritoneum ?
-
 ?
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate Rattus norvegicus
-
 [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus O35776 isozyme HAS2; isozyme HAS2
-
Rattus norvegicus Q8CH92 isozyme HAS3
-
Rattus norvegicus Q8CH93 isozyme HAS1; 3 isozymes HAS1, HAS2, and HAS3
-

Source Tissue

Source Tissue Comment Organism Textmining
3Y1 cell fibroblast, expression of isozymes HAS1 and HAS2 is increased after oncogenic malignant transformation with v-sre and/or v-fos, while only the expression of isozyme HAS2 is increased by transformation with v-HA-ras Rattus norvegicus
-
3Y1 cell fibroblast, oncogenic malignant transformation with v-sre and/or v-fos, and v-HA-ras Rattus norvegicus
-
fibroblast
-
 Rattus norvegicus
-
HR-3Y1 cell tumorigenic cell line, 3-5fold higher hyaluronan accumulation than in the parental cell line 3Y1 Rattus norvegicus
-
peritoneum
-
 Rattus norvegicus
-
SR-3Y1 cell metastatic cell line, 3-5fold higher hyaluronan accumulation than in the parental cell line 3Y1 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity in wild-type cells and their oncogenic transformants Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isozyme expression and effects on tumor development and growth in rats, overview Rattus norvegicus ?
-
 ?
additional information isozyme expression and effects on tumor development and growth in rats, overview, repression of HAS2 expression leads to reduced hyaluronan synthesis and reduced tumorigenicity in the peritoneum Rattus norvegicus ?
-
 ?
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate
-
 Rattus norvegicus [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
 ?
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate
-
 Rattus norvegicus [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP product is a linear chain ?

Synonyms

Synonyms Comment Organism
HAS
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 assay at Rattus norvegicus