Literature summary for 2.4.1.210 extracted from

Devaiah, S.P.; Owens, D.K.; Sibhatu, M.B.; Sarkar, T.R.; Strong, C.L.; Mallampalli, V.K.; Asiago, J.; Cooke, J.; Kiser, S.; Lin, Z.; Wamucho, A.; Hayford, D.; Williams, B.E.; Loftis, P.; Berhow, M.; Pike, L.M.; McIntosh, C.A.
Identification, recombinant expression, and biochemical analysis of putative secondary product glucosyltransferases from Citrus paradisi (2016), J. Agric. Food Chem., 64, 1957-1969.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene SLLGT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli and in Pichia pastoris	Citrus x paradisi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Citrus x paradisi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucose + limonin	Citrus x paradisi	-	glucosyl-limonin + UDP	-	?

Organism

Organism	UniProt	Comment	Textmining
Citrus x paradisi	B2YGX8	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli and from Pichia pastoris	Citrus x paradisi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + limonin	-	Citrus x paradisi	glucosyl-limonin + UDP	-	?
UDP-glucose + limonoate A-ring-lactone	-	Citrus x paradisi	UDP + limonin 17-beta-D-glucoside	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Citrus x paradisi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	-	assay at	Citrus x paradisi

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to clade C of flavonoid and limonoid glucosyltransferases	Citrus x paradisi

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Release 2023.1 (January 2023)