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Literature summary for 2.4.1.21 extracted from

  • Asencion Diez, M.D.; Peiru, S.; Demonte, A.M.; Gramajo, H.; Iglesias, A.A.
    Characterization of recombinant UDP- and ADP-glucose pyrophosphorylases and glycogen synthase to elucidate glucose-1-phosphate partitioning into oligo- and polysaccharides in Streptomyces coelicolor (2012), J. Bacteriol., 194, 1485-1493.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His-tagged GSase in Escherichia coli strain BL21(DE3) or Top10 Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
Co2+ over 90% inhibition Streptomyces coelicolor
Cu2+ over 90% inhibition Streptomyces coelicolor
Ni2+ over 90% inhibition Streptomyces coelicolor
Zn2+ over 90% inhibition Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.5fold activation at 10 mM Streptomyces coelicolor
Mn2+ 10 mM, 47% of the activity with Mg2+ Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-glucose + [(1->4)-alpha-D-glucosyl]n Streptomyces coelicolor glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
ADP-glucose + [(1->4)-alpha-D-glucosyl]n Streptomyces coelicolor A3(2) glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged GSase from Escherichia coli strain BL21(DE3) or Top10 by nickel affinity chromatography and dialysis Streptomyces coelicolor

Storage Stability

Storage Stability Organism
-80°C, purified His-tagged enzyme, in 20 mM triethanolamine-HCl, pH 8.0, and 20% v/v glycerol, stable for at least 6 months Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + [(1->4)-alpha-D-glucosyl]n glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide Streptomyces coelicolor ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
ADP-glucose + [(1->4)-alpha-D-glucosyl]n substrate is rabbit liver glycogen Streptomyces coelicolor ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
ADP-glucose + [(1->4)-alpha-D-glucosyl]n glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide Streptomyces coelicolor A3(2) ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
ADP-glucose + [(1->4)-alpha-D-glucosyl]n substrate is rabbit liver glycogen Streptomyces coelicolor A3(2) ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Subunits

Subunits Comment Organism
dimer SDS-PAGE and gel filtration, quaternary structure determination, recombinant enzyme Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
GSase
-
 Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces coelicolor

General Information

General Information Comment Organism
metabolism glycogen synthesis occurs via ADP-glucose in Streptomyces coelicolor, with the pathway being strictly regulated in connection with other routes involved with oligo- and polysaccharides, as well as with antibiotic synthesis in the bacterium, metabolic scenario for glucose 6-phosphate partitioning, overview Streptomyces coelicolor