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Literature summary for 2.4.1.21 extracted from

  • Greenberg, E.; Preiss, J.
    Biosynthesis of bacterial glycogen. II. Purification and properties of the adenosine diphosphoglucose:glycogen transglucosylase of Arthrobacter specise NRRL B1973 (1965), J. Biol. Chem., 240, 2341-2348.
    View publication on PubMed
Search for more literature for 2.4.1.21

Activating Compound

Activating Compound Comment Organism Structure
Bovine plasma albumin activation Arthrobacter sp.
glutathione activation Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Arthrobacter sp.
p-chloromercuribenzoate 0.002 mM, 50% inhibition, 0.01 mM, 90% inhibition Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Arthrobacter sp.
0.000164
-
 ADPglucose
-
 Arthrobacter sp.
0.000272
-
 dADPglucose
-
 Arthrobacter sp.
0.000864
-
 glycogen Arthrobacter glycogen Arthrobacter sp.
0.015
-
 maltotriose
-
 Arthrobacter sp.
0.077
-
 maltose
-
 Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Arthrobacter sp.
-
-
starch grain
-
 Arthrobacter sp. 43036
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 20-30% activation Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 NRRL B1973
-
Arthrobacter sp. NRRL B1973
-
 NRRL B1973
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, calcium phosphate gel, DEAE-cellulose Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.93
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + alpha-1,4-polyglucan citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptors: Arthrobacter glycogen, potato starch, maltose and maltotriose Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylose Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylopectin Arthrobacter sp. ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptors: Arthrobacter glycogen, potato starch, maltose and maltotriose Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylose Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylopectin Arthrobacter sp. NRRL B1973 ADP + alpha-1,4-polyglucan
-
 ?
ADP-glucose + glycogen
-
 Arthrobacter sp. ADP + ?
-
 ?
ADP-glucose + maltose
-
 Arthrobacter sp. ADP + maltotriose + maltotetraose
-
 ?
ADP-glucose + maltotriose
-
 Arthrobacter sp. ADP + maltotetraose
-
 ?
dADPglucose + alpha-1,4-polyglucan
-
 Arthrobacter sp. dADP + alpha-1,4-polyglucan
-
 ?
dADPglucose + alpha-1,4-polyglucan
-
 Arthrobacter sp. NRRL B1973 dADP + alpha-1,4-polyglucan
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 Arthrobacter sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.08
-
 ADP
-
 Arthrobacter sp.