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Literature summary for 2.4.1.21 extracted from
- Soluble adenosine diphosphate glucose alpha-1,4-glucan alpha-4-glucosyltransferases from spinach leaves (1972), Biochem. J., 126, 953-963.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | amylose: 0.02-0.06 mg/ml, isoenzymes I, II and IV, amylopectin: 0.03-0.05 mg/ml, isoenzymes I, II and IV, rabbit liver glycogen: 0.8-1.02 mg/ml, isoenzymes I, II and IV, oyster glycogen: 1.18-6.66 mg/ml, isoenzymes I-IV, amylopectin beta-limit dextrin: 0.02-0.22 mg/ml, isoenzymes I-IV, glycogen E. coli beta-limit dextrin: 1.0-2.0 mg/ml, isoenzymes I-IV | Spinacia oleracea | |
| 0.15 | - |
ADPglucose | isoenzyme III | Spinacia oleracea |  |
| 0.2 | - |
ADPglucose | isoenzyme I | Spinacia oleracea | |
| 0.25 | - |
ADPglucose | isoenzyme IV | Spinacia oleracea | |
| 0.29 | - |
ADPglucose | isoenzyme II | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Spinacia oleracea | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
isoenzymes I-IV | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4 isoenzymes, ammonium sulfate, DEAE-cellulose | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.032 | - |
primed reaction, isoenzyme I | Spinacia oleracea |
| 0.106 | - |
primed reaction, isoenzyme IV | Spinacia oleracea |
| 0.118 | - |
primed reaction, isoenzyme III | Spinacia oleracea |
| 0.28 | - |
unprimed reaction, isoenzyme III | Spinacia oleracea |
| 0.338 | - |
primed reaction, isoenzyme II | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + alpha-1,4-polyglucan | citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen | Spinacia oleracea | ADP + alpha-1,4-polyglucan | - |
? | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptor: oyster glycogen | Spinacia oleracea | ADP + alpha-1,4-polyglucan | - |
? | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptors: amylopectin beta-limit dextrin, glycogen E. coli beta-limit dextrin, maltose and maltotriose | Spinacia oleracea | ADP + alpha-1,4-polyglucan | - |
? | |
| ADP-glucose + alpha-1,4-polyglucan | 4 isoenzymes, isoenzyme III shows unprimed activity, no activity with UDPglucose and glucose 1-phosphate | Spinacia oleracea | ADP + alpha-1,4-polyglucan | - |
? | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptor: amylose | Spinacia oleracea | ADP + alpha-1,4-polyglucan | - |
? | |
| ADP-glucose + alpha-1,4-polyglucan | glucosyl acceptor: amylopectin | Spinacia oleracea | ADP + alpha-1,4-polyglucan | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
4 isozymes, primed reaction | Spinacia oleracea |
| 37 | - |
unprimed synthase activity of isoenzyme III | Spinacia oleracea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | 44 | - |
Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | isoenzymes I-IV | Spinacia oleracea |
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Release 2023.1 (January 2023)
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