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Literature summary for 2.4.1.209 extracted from

  • Rasmussen, S.; Rudolph, H.
    Isolation, purification and characterization of UDP-glucose:cis-p-coumaric acid-beta-D-glucosyltransferase from Sphagnum fallax (1997), Phytochemistry, 46, 449-453.
No PubMed abstract available
Search for more literature for 2.4.1.209

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 14 mM, 30% inhibition Sphagnum fallax
cis-p-coumaric acid inhibits the rection above 1 mM concentration Sphagnum fallax
NaCl 0.4 M, 40% inhibition Sphagnum fallax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 cis-p-coumarate
-
 Sphagnum fallax
0.11
-
 UDP-glucose cosubstrate Sphagnum fallax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 gel filtration Sphagnum fallax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + cis-coniferyl alcohol + H2O Fagus grandifolia
-
 ?
-
 ?
UDP-glucose + cis-p-coumarate + H2O Sphagnum fallax
-
 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
-
 ?

Organism

Organism UniProt Comment Textmining
Fagus grandifolia
-
 american beech
-
Sphagnum fallax
-
 peat moss
-

Purification (Commentary)

Purification (Comment) Organism
partially, 600fold Sphagnum fallax

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.054
-
-
 Sphagnum fallax

Storage Stability

Storage Stability Organism
-18°C, freezing results in a loss of activity of more than 50% Sphagnum fallax
4°C, loses more than 25% of its activity within 24 h, 5% glycerol causes total loss of activity after 48 h Sphagnum fallax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-caffeic acid + UDP-glucose + H2O enzyme activity of 10% compared with that using cis-p-coumaric acid as substrate Sphagnum fallax 4'-O-beta-D-glucosyl-cis-caffeate + UDP
-
 ?
cis-p-coumarate + UDP-glucose + H2O
-
 Sphagnum fallax 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
-
 ?
UDP-glucose + cis-coniferyl alcohol + H2O
-
 Fagus grandifolia ?
-
 ?
UDP-glucose + cis-p-coumarate
-
 Sphagnum fallax 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
-
 ?
UDP-glucose + cis-p-coumarate + H2O
-
 Sphagnum fallax 4'-O-beta-D-glucosyl-cis-p-coumarate + UDP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
 100 mM glycine KOH Sphagnum fallax

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.3
-
 Sphagnum fallax

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.8 9.5 below pH 7.8 and above pH 9.5 rapid drop in enzyme activity Sphagnum fallax