Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus tremuloides | - |
gene PtxtXET16-34 | - |
Synonyms | Comment | Organism |
---|---|---|
xyloglucan endo-transglycosylase | - |
Populus tremula x Populus tremuloides |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the GH16 XTH family, extension clan GH-B enzymes | Populus tremula x Populus tremuloides |
additional information | catalytic nucleophile is Glu85, general acid/base residue is Glu89, Asp87 is the so-called helper residue, molecular dynamics simulations of a branched tetradecasaccharide substrate XXXGXXXG in the active site of xyloglucan endo-transglycosylase. When Asp87 is deprotonated, electrostatic repulsion forces the nucleophilic away from C1 of the sugar ring in subsite 21 and the proton-donating ability of Glu89 iss also weakened due to the formation of a hydrogen bond with Asp87, whereas protonation of Asp87 results in the formation of a hydrogen bond with the catalytic nucleophile and correct positioning of the catalytic machinery | Populus tremula x Populus tremuloides |