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Literature summary for 2.4.1.207 extracted from
- Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.) (2009), FEBS J., 276, 437-456.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Hordeum vulgare |
General Stability
| General Stability | Organism |
|---|---|
| repeated cycles of freezing and thawing reduce its activity to around 10% | Hordeum vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0085 | - |
reduced xyloglucan-derived heptasaccharide | recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare | |
| 0.0399 | - |
reduced xyloglucan-derived heptasaccharide | purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare | |
| 1.6 | - |
donor Glc8-based xyloglucan oligosaccharide | recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | 35000 | SDS-PAGE shows a single, but somewhat diffuse band of 33-35 kDa, indicating that the protein could be glycosylated | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | B1P1S7 | isozyme XET6 | - |
| Hordeum vulgare | P93668 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography and Bio-Gel P60 gel filtration | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Hordeum vulgare | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.072 | - |
enzyme from crude homogenate | Hordeum vulgare |
| 31.07 | - |
after 432fold purification | Hordeum vulgare |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or -80°C, activity declines gradually | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor Glc8-based xyloglucan oligosaccharide + acceptor sulforhodamine-labelled xyloglucan oligosaccharide | - |
Hordeum vulgare | ? | - |
? |  |
| Glc8-based xyloglucan oligosaccharide + ? | - |
Hordeum vulgare | ? | - |
? | |
| hydroxyethyl cellulose + reduced xyloglucan-derived heptasaccharide | 3.4% activity compared to tamarind xyloglycan | Hordeum vulgare | ? | - |
? | |
| additional information | no activity towards barley 1,3-1,4-beta-D-glucan (high viscosity), and locust bean gum galactomannan, no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin and esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fibre rhamnogalactouronan, beta-D-galactans (from lupin and potato) and arabinogalactan protein (from gum arabic) | Hordeum vulgare | ? | - |
? | |
| additional information | no hydrolytic activity can be detected with either isoenzyme | Hordeum vulgare | ? | - |
? | |
| pea xyloglucan + reduced xyloglucan-derived heptasaccharide | - |
Hordeum vulgare | ? | - |
? | |
| tamarind xyloglucan + reduced xyloglucan-derived heptasaccharide | 100% activity | Hordeum vulgare | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XET | - |
Hordeum vulgare |
| XET5 | isozyme | Hordeum vulgare |
| XET6 | isozyme | Hordeum vulgare |
| xyloglucan endotransglycosylase | - |
Hordeum vulgare |
| xyloglucan xyloglucosyl transferase | - |
Hordeum vulgare |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
about 25% of isozyme XET6 activity survives incubation at 95°C for 1 min | Hordeum vulgare |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0024 | - |
reduced xyloglucan-derived heptasaccharide | purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare | |
| 0.0089 | - |
reduced xyloglucan-derived heptasaccharide | recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare | |
| 0.139 | - |
Glc8-based xyloglucan oligosaccharide | recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hordeum vulgare | isoelectric focusing | - |
6.7 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0869 | - |
Glc8-based xyloglucan oligosaccharide | recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare | |
| 0.2 | - |
reduced xyloglucan-derived heptasaccharide | purified isozyme XET5, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare | |
| 1.1 | - |
reduced xyloglucan-derived heptasaccharide | recombinant isozyme XET6, at 30°C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2 | Hordeum vulgare |
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