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Literature summary for 2.4.1.207 extracted from
- Production of poplar xyloglucan endotransglycosylase using the methylotrophic yeast Pichia pastoris (2005), Appl. Biochem. Biotechnol., 126, 61-77.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Populus tremula x Populus tremuloides |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
SDS-PAGE | Populus tremula x Populus tremuloides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tremula x Populus tremuloides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SP-Sepharose fast flow column chromatography and S-source 15S gel filtration | Populus tremula x Populus tremuloides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
after purification the concentration of enzymatically active XET is about 29 mg/l with a specific activity of 11 U/mg | Populus tremula x Populus tremuloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + acceptor xyloglucan | - |
Populus tremula x Populus tremuloides | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XET | - |
Populus tremula x Populus tremuloides |
| xyloglucan endotransglycosylase | - |
Populus tremula x Populus tremuloides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
- |
Populus tremula x Populus tremuloides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Populus tremula x Populus tremuloides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Populus tremula x Populus tremuloides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4 | at pH 3.5-4.0 no degradation is observed | Populus tremula x Populus tremuloides |
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Release 2023.1 (January 2023)
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