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Literature summary for 2.4.1.207 extracted from

  • Henriksson, H.; Denman, S.E.; Campuzano, I.D.; Ademark, P.; Master, E.R.; Teeri, T.T.; Brumer, H.
    N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A (2003), Biochem. J., 375, 61-73.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information glycosylation is required for activity, deglycosylation results in 40% loss of activity Brassica oleracea var. botrytis

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Pichia pastoris resulting in a recombinant enzyme which is more highly mannosylated than the native enzyme without having influence on activity Brassica oleracea var. botrytis

General Stability

General Stability Organism
about 30% activity is lost during one freeze-thaw cycle Brassica oleracea var. botrytis

Inhibitors

Inhibitors Comment Organism Structure
additional information glycosylation is required for activity, deglycosylation results in 40% loss of activity Brassica oleracea var. botrytis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31737
-
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry Brassica oleracea var. botrytis
33115
-
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry Brassica oleracea var. botrytis

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. botrytis Q6YDN9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein one N-glycosylation site situated close to the predicted catalytic residues, enzyme is a high-mannose-type glycan, glycosylation is required for activity, deglycosylation results in 40% loss of activity, overview Brassica oleracea var. botrytis

Purification (Commentary)

Purification (Comment) Organism
native enzyme from florets to homogeneity by adsorption chromatography, 55-140fold purification dependent on different assay methods for quantification Brassica oleracea var. botrytis

Reaction

Reaction Comment Organism Reaction ID
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc enzyme possesss the catalytic sequence motif EIDFE conserved in the glycosyl hydrolase family 16 Brassica oleracea var. botrytis

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
Brassica oleracea var. botrytis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
different assay methods, overview Brassica oleracea var. botrytis
0.812
-
purified enzyme, radioactive assay method Brassica oleracea var. botrytis

Storage Stability

Storage Stability Organism
14°C, 0.1 mg/ml enzyme in 50 mM ammonium acetate, pH 5.5, with 0.5 M ammonium sulfate, complete loss of activity within 3 months Brassica oleracea var. botrytis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information different assay methods, overview Brassica oleracea var. botrytis ?
-
?
xyloglucan oligosaccharides + xyloglucan
-
Brassica oleracea var. botrytis ?
-
?

Subunits

Subunits Comment Organism
? x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry Brassica oleracea var. botrytis

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16 Brassica oleracea var. botrytis
XET
-
Brassica oleracea var. botrytis
xyloglucan endotransglycosylase 16A
-
Brassica oleracea var. botrytis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Brassica oleracea var. botrytis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 about 20% of maximal activity at 10°C and 50°C, about 50% of maximal activity at 20°C and 48°C, temperature-profile Brassica oleracea var. botrytis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Brassica oleracea var. botrytis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH-profile, sigmoidal decline in activity at high pH, at low pH occurs an abrupt drop in activity Brassica oleracea var. botrytis