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Literature summary for 2.4.1.207 extracted from
- In vitro activities of four xyloglucan endotransglycosylases from Arabidopsis (1999), Plant J., 18, 371-382.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and expression of TCH4, Meri-5, EXGT and XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced | Arabidopsis thaliana |
| cloning and expression of XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | significant substrate inhibition of isoenzyme XTR9 at high concentrations; slight substrate inhibition of isoenzymes Meri-5 and EXGT at high concentrations | Arabidopsis thaliana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme XTR9 | Arabidopsis thaliana | |
| 0.017 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme TCH4 | Arabidopsis thaliana | |
| 0.03 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme EXGT | Arabidopsis thaliana | |
| 0.04 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme Meri-5 | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + xyloglucan acceptor | Arabidopsis thaliana | XETs encoded by a gene family may influence plant growth and development, low pH would limit XET function in vivo | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
XETs are encoded by a gene family, 4 isoenzymes: TCH4, Meri-5, EXGT and XTR9 | - |
| Arabidopsis thaliana | Q9ZSU4 | XTR9, one of 4 isoenzymes: TCH4, Meri-5, EXGT and XTR9 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | all 4 isoenzymes, TCH4, Meri-5, EXGT and XTR9, are N-glycosylated, requirement of glycosylation for activity of the isoenzymes differs | Arabidopsis thaliana |
| additional information | co- and post-translational eucaryotic-specific modifications of TCH4 are critical for optimal XET activity | Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, recombinant TCH4, Meri-5, EXGT and XTR9, expressed in Sf9 insect cells | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + acceptor xyloglucan | acceptor and donor substrate specificities of the isoenzymes TCH4, Meri-5, EXGT and XTR9 | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | isoenzyme XTR9 has a clear preference for non-fucosylated xyloglucan polymers as donor, but not isoenzymes TCH4, Meri-5 and EXGT | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol | XXFG-ol | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol | TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide, TCH4, Meri-5 and EXGT: 25-30% as effective as XLLG-ol, less effective than XXXG-ol, XTR9: more effective than XXXG-ol, donor: tamarind or pea xyloglucan polymer | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | donor: tamarind or pea xyloglucan polymer | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XLLG-ol | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide, TCH4, Meri-5 and EXGT: more effective than XXFG-ol, XTR9: less effective than XXFG-ol, donor: tamarind or pea xyloglucan polymer | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XXXG-ol | Arabidopsis thaliana | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | XETs encoded by a gene family may influence plant growth and development, low pH would limit XET function in vivo | Arabidopsis thaliana | ? | - |
? | |
| additional information | contains strongly conserved sequence motif of XETs: DEIDFEFLG | Arabidopsis thaliana | ? | - |
? | |
| additional information | no endoglucanase activity | Arabidopsis thaliana | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Arabidopsis thaliana |
| 18 | - |
isoenzymes TCH4 and XTR9 | Arabidopsis thaliana |
| 28 | - |
isoenzyme Meri-5 | Arabidopsis thaliana |
| 37 | - |
isoenzyme EXGT | Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
all 4 isoenzymes are markedly cold-tolerant retaining activity at 5°C | Arabidopsis thaliana |
| 45 | - |
isoenzymes TCH4 and XTR9 activities are reduced by 55-60% | Arabidopsis thaliana |
| 45 | - |
isoenzymes EXGT and Meri-5 retain approximately 80% of maximum activity | Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
all 4 isoenzymes are markedly cold-tolerant retaining activity at 5°C | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | isoenzymes TCH4, Meri-5, EXGT and XTR9, EXGT shows very sharp pH-optimum at pH 6 | Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | XTR9 retains approximately 35% of its maximal activity at pH 4.5 and 30% at pH 8 | Arabidopsis thaliana |
| 4.5 | 8 | TCH4 and Meri-5 retain approximately 10-15% of its maximal activity at pH 4.5 and 65% at pH 8 | Arabidopsis thaliana |
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