Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.201 extracted from

  • Schachter, H.; Brockhausen, I.; Hull, E.
    High-performance liquid chromatography assays for N-acetylglucosaminyltransferases involved in N- and O-glycan synthesis (1989), Methods Enzymol., 179, 351-397.
    View publication on PubMed
Search for more literature for 2.4.1.201

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation, 0.125-1.25% Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Gallus gallus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation, 12.5 mM, can substitute partially for Mn2+ Gallus gallus
Co2+ activation, 12.5 mM Gallus gallus
Co2+ can substitute partially for Mn2+ Gallus gallus
Mg2+ activation, 12.5 mM Gallus gallus
Mg2+ can substitute partially for Mn2+ Gallus gallus
Mn2+ absolute requirement Gallus gallus
Mn2+ maximal activity at 0.05-0.1 M Gallus gallus
additional information no activation by 12.5 mM Cd2+, Ni2+ or Zn2+ Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R Gallus gallus involved in N-glycan biosynthesis UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 hen
-

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R R represents the remainder of the N-oligosaccharide core of asparagine-linked glycopeptides, (+/-)-fucose, minimum structure requirement: N-acetyl-D-glucosaminyl-beta-1,6-(N-acetylglucosaminyl-beta-1,2)-alpha-mannosyl-R Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R acts on bisected and non-bisected substrates Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R compounds lacking beta-2-linked N-acetylglucosamine residues are no acceptor substrates Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R involved in N-glycan biosynthesis Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.4
-
 Gallus gallus