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Literature summary for 2.4.1.2 extracted from
- A novel dextran dextrinase from Gluconobacter oxydans DSM-2003: purification and properties (2012), Appl. Biochem. Biotechnol., 168, 1256-1264.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Gluconobacter oxydans |  |
| Cu2+ | strong inhibition | Gluconobacter oxydans | |
| Fe2+ | strong inhibition | Gluconobacter oxydans | |
| Ni2+ | - |
Gluconobacter oxydans | |
| Zn2+ | strong inhibition | Gluconobacter oxydans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | slight activation | Gluconobacter oxydans | |
| Ca2+ | slight activation | Gluconobacter oxydans | |
| Mg2+ | slight activation | Gluconobacter oxydans | |
| Mn2+ | slight activation | Gluconobacter oxydans | |
| additional information | no effect by Fe3+ | Gluconobacter oxydans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Gluconobacter oxydans | DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation | ? | - |
? | |
| additional information | Gluconobacter oxydans DSM 2003 | DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation | ? | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m | Gluconobacter oxydans | - |
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 | dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points | ? | |
| [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m | Gluconobacter oxydans DSM 2003 | - |
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 | dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
| Gluconobacter oxydans DSM 2003 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native DDase 187.5fold to homogeneity by anion exchange chromatography and gel filtration | Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 112.5 | - |
purified enzyme, pH 6.5, 30°C | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-D-glucopyranoside + H2O | artificial substrate | Gluconobacter oxydans | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| 4-nitrophenyl-alpha-D-glucopyranoside + H2O | artificial substrate | Gluconobacter oxydans DSM 2003 | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| additional information | DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation | Gluconobacter oxydans | ? | - |
? | |
| additional information | DDase from strain DSM-2003 converts maltodextrins to dextran. The enzyme catalyzes the transfer of an alpha(1,4)-linked glucosyl unit from a donor to an acceptor molecule, forming an alpha(1,6) linkage: consecutive glucosyl transfers result in the formation of high molecular weight dextran from maltodextrin. DDase can use widely occurring in nature maltodextrin to produce dextran by transglucosylation | Gluconobacter oxydans DSM 2003 | ? | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m | - |
Gluconobacter oxydans | [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m | - |
Gluconobacter oxydans | [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 | dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points | ? | |
| [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m | - |
Gluconobacter oxydans DSM 2003 | [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 | - |
? | |
| [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m | - |
Gluconobacter oxydans DSM 2003 | [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 | dextran synthesized by DDase from strain DSM-2003 is composed of alpha(1,6)-linked chains containing alpha(1,4) branching points | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, SDS-PAGE | Gluconobacter oxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DDase | - |
Gluconobacter oxydans |
| dextran dextrinase | - |
Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Gluconobacter oxydans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 11 | 35 | sharp decrease at temperatures higher than 35 °C | Gluconobacter oxydans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below 30°C for 3 h | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Gluconobacter oxydans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | stable within this range for 3 h | Gluconobacter oxydans |
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