Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 0.5 mM, 83% inhibition | Gluconobacter oxydans | |
additional information | not inhibited by Ca2+, Co2+, Fe2+, Mg2+, Mn2+, Ni2+, Zn2+, p-chloromercuribenzoate and EDTA | Gluconobacter oxydans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | slight activation | Gluconobacter oxydans | |
additional information | no activation by Ca2+, Co2+, Fe2+, Fe3+, Mg2+, Ni2+, Zn2+ and EDTA | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
x * 300000, SDS-PAGE | Gluconobacter oxydans |
Organic Solvent | Comment | Organism |
---|---|---|
Ethylene glycol | 40% v/v in acetate buffer, stable to | Gluconobacter oxydans |
n-Butanol | H2O-saturated, 50% v/v in acetate buffer, stable to | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
ATCC 11894 | - |
Purification (Comment) | Organism |
---|---|
phenyl-Toyopearl, gel filtration | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.8 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m | maltopentaose, maltohexaose, short-chain amylose, soluble starch | Gluconobacter oxydans | (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1 | - |
? | |
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m | substrates are maltooligosaccharides, e.g. maltotriose (G3), maltotetraose (G4) | Gluconobacter oxydans | (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1 | - |
? | |
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m | poor substrate: maltose (G2) | Gluconobacter oxydans | (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1 | - |
? | |
maltohexaose | - |
Gluconobacter oxydans | dextran | dextran yield 30.2% | ? | |
maltopentaose | - |
Gluconobacter oxydans | dextran | dextran yield 25.0% | ? | |
maltotetraose | - |
Gluconobacter oxydans | dextran | dextran yield 13.4% | ? | |
maltotriose | - |
Gluconobacter oxydans | dextran | dextran yield 11.0% | ? | |
short-chain amylose | - |
Gluconobacter oxydans | dextran | dextran yield 57.6% | ? | |
soluble starch | - |
Gluconobacter oxydans | dextran | dextran yield 21.4% | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 300000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Gluconobacter oxydans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
17 | 52 | approx. half-maximal activity at 17°C and 52°C | Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, stable below | Gluconobacter oxydans |
55 | - |
inactivation within 30 min | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.2 | broad optimum | Gluconobacter oxydans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5.7 | about half-maximal activity at pH 3.0 and 5.7 | Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.2 | 30 min stable | Gluconobacter oxydans |