Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | strong inhibition in the presence of Mg2+ and Mn2+ | Dictyostelium discoideum | |
EDTA | strong | Dictyostelium discoideum | |
EGTA | strong | Dictyostelium discoideum | |
Mg2+ | strong | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | 0.43 | O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine | i.e. Man5GlcNAc, depending whether N-acetyl-D-glucosamine residue is intersected or bisected | Dictyostelium discoideum | |
0.65 | - |
O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine | i.e. Man9GlcNAc, intersected product | Dictyostelium discoideum | |
1.2 | - |
UDP-N-acetyl-D-glucosamine | cosubstrate Man9GlcNAc | Dictyostelium discoideum | |
1.42 | - |
methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside | intersected product | Dictyostelium discoideum | |
2.54 | - |
O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine | bisected product | Dictyostelium discoideum | |
18.52 | - |
O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl | intersected product | Dictyostelium discoideum | |
19.48 | - |
O-mannopyranosyl-alpha-1,3-mannopyranosyl-beta-1,4-N-acetyl-glucosamine | bisected product | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-associated | Dictyostelium discoideum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | stimulation | Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain M4, partial | Dictyostelium discoideum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000077 | - |
Man5GlcNAc, intersected | Dictyostelium discoideum |
0.0000129 | - |
Man9GlcNAc, intersected | Dictyostelium discoideum |
0.0000133 | - |
Man5GlcNAc, bisected | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + (O-mannopyranosyl-alpha-1,3)-mannopyranosyl-beta-1,4-N-acetyl-glucosamine | i.e. Man2GlcNAc, very low activity | Dictyostelium discoideum | UDP + O-mannopyranosyl-alpha-1,3-[N-acetyl-glucosamine-beta-1,4]-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine | - |
? | |
UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside | trivial name Man3Me | Dictyostelium discoideum | UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine | - |
? | |
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine | i.e. Man9GlcNAc oligosaccharide, enzyme catalyzes the transfer of N-acetyl-glucosamine to both alpha- and beta linked mannoses | Dictyostelium discoideum | UDP + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-(N-acetyl-D-glucosamine-beta-1,4)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine | i.e. Man9GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue attached beta-1,4 to the mannose linked alpha-1,6 to the core mannose in an intersecting position | ? | |
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl | i.e. Man2Me | Dictyostelium discoideum | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine | i.e. Man5GlcNAc oligosaccharide, 20% of activity compared to Man9GlcNAc | Dictyostelium discoideum | UDP + O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine | i.e. Man5GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue in an intersected and/or bisected position, so that either the core beta-mannose or the branching alpha-mannose residue accepts the transferred N-acetyl-D-glucosamine residue | ? | |
UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine | i.e. Man3GlcNAc | Dictyostelium discoideum | UDP + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6-(N-acetyl-D-glucosamine beta-1,4)]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Dictyostelium discoideum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
75% decrease of activity after 2 min | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.9 | - |
Dictyostelium discoideum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.2 | 20% of maximal activity at pH 5.5, 70% of maximal activity at pH 7.2 | Dictyostelium discoideum |