Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paenibacillus illinoisensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 68% residual activity at 10 mM | Paenibacillus illinoisensis | |
Cu2+ | 25% residual activity at 10 mM | Paenibacillus illinoisensis | |
Hg2+ | complete inhibition at 1 mM | Paenibacillus illinoisensis | |
Mg2+ | 89% residual activity at 10 mM | Paenibacillus illinoisensis | |
additional information | not inhibited by EDTA | Paenibacillus illinoisensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activating effect (113% activity at 10 mM) | Paenibacillus illinoisensis | |
additional information | Ni2+ and Na+ ions have weak effects on the enzyme activity | Paenibacillus illinoisensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
x * 74000, SDS-PAGE | Paenibacillus illinoisensis |
74352 | - |
x * 74352, calculated from amino acid sequence | Paenibacillus illinoisensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + glycosyl acceptor | Paenibacillus illinoisensis | - |
beta-cyclodextrin | - |
? | |
soluble starch + glycosyl acceptor | Paenibacillus illinoisensis ZY-08 | - |
beta-cyclodextrin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus illinoisensis | - |
- |
- |
Paenibacillus illinoisensis ZY-08 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Paenibacillus illinoisensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the native enzyme does not convert king oyster mushroom powder and enoki mushroom powder, while recombinant enzyme converts king oyster mushroom powder and enoki mushroom powder to beta-cyclodextrin | Paenibacillus illinoisensis | ? | - |
? | |
additional information | the native enzyme does not convert king oyster mushroom powder and enoki mushroom powder, while recombinant enzyme converts king oyster mushroom powder and enoki mushroom powder to beta-cyclodextrin | Paenibacillus illinoisensis ZY-08 | ? | - |
? | |
soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis | beta-cyclodextrin | - |
? | |
soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis ZY-08 | beta-cyclodextrin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 74000, SDS-PAGE | Paenibacillus illinoisensis |
? | x * 74352, calculated from amino acid sequence | Paenibacillus illinoisensis |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Paenibacillus illinoisensis |
cyclodextrin glucanotransferase | - |
Paenibacillus illinoisensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Paenibacillus illinoisensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | about 65% activity at 20°C, around 95-100% activity at 30-50°C, about 50% activity at 60°C | Paenibacillus illinoisensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | the enzyme remains fully stable between 20 and 40°C for 30 min, while at 50°C activity decreases to about 65% and is lost at 60°C | Paenibacillus illinoisensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Paenibacillus illinoisensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | more than 60% activity between pH 5.0 and 9.0 | Paenibacillus illinoisensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the enzyme is stable from pH 6.0 to 9.0 for 30 min with a gradual loss of activity at higher and lower pH values in the range of 6.0-9.0 | Paenibacillus illinoisensis |