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Literature summary for 2.4.1.19 extracted from

  • Ara, K.Z.; Lundemo, P.; Fridjonsson, O.H.; Hreggvidsson, G.O.; Adlercreutz, P.; Karlsson, E.N.
    A CGTase with high coupling activity using gamma-cyclodextrin isolated from a novel strain clustering under the genus Carboxydocella (2015), Glycobiology, 25, 514-523.
    View publication on PubMed
Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells uncultured Carboxydocella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE uncultured Carboxydocella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
potato starch + glycosyl acceptor uncultured Carboxydocella sp. worst substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
potato starch + glycosyl acceptor uncultured Carboxydocella sp. MAT3016 worst substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor uncultured Carboxydocella sp.
-
 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor uncultured Carboxydocella sp. MAT3016
-
 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
wheat starch + glycosyl acceptor uncultured Carboxydocella sp. best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
wheat starch + glycosyl acceptor uncultured Carboxydocella sp. MAT3016 best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured Carboxydocella sp.
-
-
-
uncultured Carboxydocella sp. MAT3016
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, and gel filtration uncultured Carboxydocella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
potato starch + glycosyl acceptor worst substrate uncultured Carboxydocella sp. alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
potato starch + glycosyl acceptor worst substrate uncultured Carboxydocella sp. MAT3016 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor
-
 uncultured Carboxydocella sp. alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor
-
 uncultured Carboxydocella sp. MAT3016 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
wheat starch + glycosyl acceptor best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate uncultured Carboxydocella sp. alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
wheat starch + glycosyl acceptor best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate uncultured Carboxydocella sp. MAT3016 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE uncultured Carboxydocella sp.

Synonyms

Synonyms Comment Organism
CGT13
-
 uncultured Carboxydocella sp.
CGTase
-
 uncultured Carboxydocella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 uncultured Carboxydocella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 uncultured Carboxydocella sp.