Cloned (Comment) | Organism |
---|---|
mutant Y167H is expressed in Escherichia coli BL21(DE3) cells | Paenibacillus macerans |
Crystallization (Comment) | Organism |
---|---|
mutant Y167H, hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.05 M Tris-HCl, 0.1 M sodium acetate buffer, 25 mM Na2HPO4, 150 mM NaCl, 10 mM imidazole pH 8.5 | Paenibacillus macerans |
Protein Variants | Comment | Organism |
---|---|---|
Y167H | the mutant shows enhanced alpha-cyclodextrin specificity | Paenibacillus macerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Paenibacillus macerans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + glycosyl acceptor | Paenibacillus macerans | - |
cyclodextrins | - |
? | |
starch + glycosyl acceptor | Paenibacillus macerans 602-1 | - |
cyclodextrins | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | S5ZJ19 | - |
- |
Paenibacillus macerans 602-1 | S5ZJ19 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant Y167H with ammonium sulfate precipitation and Ni-NTA agarose column chromatography | Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
? | |
starch + glycosyl acceptor | - |
Paenibacillus macerans 602-1 | cyclodextrins | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Paenibacillus macerans |
Synonyms | Comment | Organism |
---|---|---|
alpha-cyclodextrin glucanotransferase | - |
Paenibacillus macerans |
CGTase | - |
Paenibacillus macerans |