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Literature summary for 2.4.1.19 extracted from

  • Gaston, J.; Szerman, N.; Costa, H.; Krymkiewicz, N.; Ferrarotti, S.
    Cyclodextrin glycosyltransferase from Bacillus circulans DF 9R: activity and kinetic studies (2009), Enzyme Microb. Technol., 45, 36-41.
No PubMed abstract available
Search for more literature for 2.4.1.19

Inhibitors

Inhibitors Comment Organism Structure
alpha-cyclodextrin competitive inhibition Niallia circulans
beta-cyclodextrin competitive inhibition Niallia circulans
gamma-cyclodextrin competitive inhibition Niallia circulans
maltose uncompetitive inhibition Niallia circulans
additional information the enzyme shows substrate and product inhibition Niallia circulans
starch soluble potato starch or cassava starch Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Niallia circulans
1.1
-
 soluble potato starch pH 6.4, 55°C, formation of beta-cyclodextrin Niallia circulans
1.69
-
 cassava starch pH 6.4, 55°C, formation of beta-cyclodextrin Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans DF 9R
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cassava starch
-
 Niallia circulans beta-cyclodextrin
-
 ?
cassava starch
-
 Niallia circulans DF 9R beta-cyclodextrin
-
 ?
additional information formation of alpha-, beta-, and gamma-cyclodextrins from starch and matotriose Niallia circulans ?
-
 ?
additional information formation of alpha-, beta-, and gamma-cyclodextrins from starch and matotriose Niallia circulans DF 9R ?
-
 ?

Synonyms

Synonyms Comment Organism
CGTase
-
 Niallia circulans
cyclodextrin glycosyltransferase
-
 Niallia circulans
More CGTase is a member of the glycoside hydrolase family 13, also known as the alpha-amylase family Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
803
-
 soluble potato starch pH 6.4, 55°C, formation of beta-cyclodextrin Niallia circulans
1612
-
 cassava starch pH 6.4, 55°C, formation of beta-cyclodextrin Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
 assay at Niallia circulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
730
-
 soluble potato starch pH 6.4, 55°C, formation of beta-cyclodextrin Niallia circulans
950
-
 cassava starch pH 6.4, 55°C, formation of beta-cyclodextrin Niallia circulans